Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines

Jeongwu Lee, Svetlana Kotliarova, Yuri Kotliarov, Aiguo Li, Qin Su, Nicholas M. Donin, Sandra Pastorino, Benjamin W. Purow, Neil Christopher, Wei Zhang, John K. Park, Howard A. Fine

Research output: Contribution to journalArticle

1512 Scopus citations

Abstract

The concept of tumor stem cells (TSCs) provides a new paradigm for understanding tumor biology, although it remains unclear whether TSCs will prove to be a more robust model than traditional cancer cell lines. We demonstrate marked phenotypic and genotypic differences between primary human tumor-derived TSCs and their matched glioma cell lines. Unlike the matched, traditionally grown tumor cell lines, TSCs derived directly from primary glioblastomas harbor extensive similarities to normal neural stem cells and recapitulate the genotype, gene expression patterns, and in vivo biology of human glioblastomas. These findings suggest that TSCs may be a more reliable model than many commonly utilized cancer cell lines for understanding the biology of primary human tumors.

Original languageEnglish (US)
Pages (from-to)391-403
Number of pages13
JournalCancer Cell
Volume9
Issue number5
DOIs
StatePublished - May 1 2006

Keywords

  • CELLCYCLE
  • HUMDISEASE
  • STEMCELL

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

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    Lee, J., Kotliarova, S., Kotliarov, Y., Li, A., Su, Q., Donin, N. M., Pastorino, S., Purow, B. W., Christopher, N., Zhang, W., Park, J. K., & Fine, H. A. (2006). Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell, 9(5), 391-403. https://doi.org/10.1016/j.ccr.2006.03.030