Tumor Suppressive Role of an Androgen-regulated Epithelial Cell Adhesion Molecule (C-CAM) in Prostate Carcinoma Cell Revealed by Sense and Antisense Approaches

J. T. Hsieh, W. Luo, W. Song, Y. Wang, D. I. Kleinerman, N. T. Van, S. H. Lin

Research output: Contribution to journalArticle

191 Scopus citations

Abstract

We recently demonstrated that C-CAM, an epithelial-cell adhesion molecule of the immunoglobulin supergene family, could be regulated by androgen and might act as a growth repressor during differentiation of the prostatic epithelium. To define the role of C-CAM in prostatic tumor-igenesis, a tumorigenic human prostatic cancer cell line, PC-3, was trans-fected with an expression plasmid containing C-CAM1 (a C-CAM isoform). Transfected clones showed significantly lower growth rates, reduced anchorage-independent growth, and less tumorigenicity in vivo than control cells. Furthermore, transfection of an antisense vector into a nontumorigenic prostatic epithelial cell line, NbE, resulted in tumor formation in nude mice. Sublines derived from these NbE-induced tumors had lower levels of C-CAM than did control cells. These data suggest that C-CAM1 can function as a tumor suppressor in prostate tumorigenesis.

Original languageEnglish (US)
Pages (from-to)190-197
Number of pages8
JournalCancer research
Volume55
Issue number1
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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