Tumorigenic Cells Are Common in Mouse MPNSTs but Their Frequency Depends upon Tumor Genotype and Assay Conditions

Johanna Buchstaller, Paul E. McKeever, Sean J. Morrison

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Tumor-initiating cells have been suggested to be rare in many cancers. We tested this in mouse malignant peripheral nerve sheath tumors (MPNSTs) and found that 18% of primary and 49% of passaged MPNST cells from Nf1 +/-; Ink4a/Arf -/- mice formed tumors upon transplantation, whereas only 1.8% to 2.6% of MPNST cells from Nf1 +/-; p53 +/- mice did. MPNST cells of both genotypes require laminin binding to β1-integrin for clonogenic growth. Most MPNST cells from Nf1 +/-; Ink4a/Arf -/- mice expressed laminin, whereas most MPNST cells from Nf1 +/-; p53 +/- mice did not. Exogenous laminin increased the percentage of MPNST cells from Nf1 +/-; p53 +/- but not Nf1 +/-; Ink4a/Arf -/- mice that formed tumorigenic colonies. Tumor-forming potential is common among MPNST cells, but the assay conditions required to detect it vary with tumor genotype.

Original languageEnglish (US)
Pages (from-to)240-252
Number of pages13
JournalCancer Cell
Volume21
Issue number2
DOIs
Publication statusPublished - Feb 14 2012

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ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Oncology

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