Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells

Lance S. Terada, Irene R. Willingham, David M. Guidot, Gayle N. Shibao, Glenn W. Kindt, John E. Repine

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalInflammation
Volume16
Issue number1
DOIs
StatePublished - Feb 1 1992

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Terada, L. S., Willingham, I. R., Guidot, D. M., Shibao, G. N., Kindt, G. W., & Repine, J. E. (1992). Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells. Inflammation, 16(1), 13-19. https://doi.org/10.1007/BF00917511