Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells

Lance S. Terada, Irene R. Willingham, David M. Guidot, Gayle N. Shibao, Glenn W. Kindt, John E. Repine

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalInflammation
Volume16
Issue number1
DOIs
StatePublished - Feb 1992

Fingerprint

Tungsten
Brain Injuries
Endothelial Cells
Tumor Necrosis Factor-alpha
Xanthine Oxidase
Brain
Calcium Ionophores
Calcimycin

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Terada, L. S., Willingham, I. R., Guidot, D. M., Shibao, G. N., Kindt, G. W., & Repine, J. E. (1992). Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells. Inflammation, 16(1), 13-19. https://doi.org/10.1007/BF00917511

Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells. / Terada, Lance S.; Willingham, Irene R.; Guidot, David M.; Shibao, Gayle N.; Kindt, Glenn W.; Repine, John E.

In: Inflammation, Vol. 16, No. 1, 02.1992, p. 13-19.

Research output: Contribution to journalArticle

Terada, LS, Willingham, IR, Guidot, DM, Shibao, GN, Kindt, GW & Repine, JE 1992, 'Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells', Inflammation, vol. 16, no. 1, pp. 13-19. https://doi.org/10.1007/BF00917511
Terada, Lance S. ; Willingham, Irene R. ; Guidot, David M. ; Shibao, Gayle N. ; Kindt, Glenn W. ; Repine, John E. / Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells. In: Inflammation. 1992 ; Vol. 16, No. 1. pp. 13-19.
@article{fecff26e6fae4070b5556936e05e827b,
title = "Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells",
abstract = "Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.",
author = "Terada, {Lance S.} and Willingham, {Irene R.} and Guidot, {David M.} and Shibao, {Gayle N.} and Kindt, {Glenn W.} and Repine, {John E.}",
year = "1992",
month = "2",
doi = "10.1007/BF00917511",
language = "English (US)",
volume = "16",
pages = "13--19",
journal = "Inflammation",
issn = "0360-3997",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Tungsten treatment prevents tumor necrosis factor-induced injury of brain endothelial cells

AU - Terada, Lance S.

AU - Willingham, Irene R.

AU - Guidot, David M.

AU - Shibao, Gayle N.

AU - Kindt, Glenn W.

AU - Repine, John E.

PY - 1992/2

Y1 - 1992/2

N2 - Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.

AB - Exposure to recombinant human tumor necrosis factor-alpha (TNF-α) or calcium ionophore (A23187) for 4 h increased (P< 0.05) lactate dehydrogenase (LDH) release from cultured bovine brain endothelial cells (EC). In contrast, treatment with endotoxin or interleukin-1 did not increase (P> 0.05) LDH release from brain EC. Pretreatment with tungsten decreased (P< 0.05) xanthine oxidase activity in brain EC and decreased (P< 0.05) LDH release from brain EC following exposure to TNF. Our results suggest that TNF-α injures brain microvascular EC and that this effect may be mediated by xanthine oxidase.

UR - http://www.scopus.com/inward/record.url?scp=0026555030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026555030&partnerID=8YFLogxK

U2 - 10.1007/BF00917511

DO - 10.1007/BF00917511

M3 - Article

VL - 16

SP - 13

EP - 19

JO - Inflammation

JF - Inflammation

SN - 0360-3997

IS - 1

ER -