TY - JOUR
T1 - Two distinct actin networks drive the protrusion of migrating cells
AU - Ponti, A.
AU - Machacek, M.
AU - Gupton, S. L.
AU - Waterman-Storer, C. M.
AU - Danuser, G.
PY - 2004/9/17
Y1 - 2004/9/17
N2 - Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.
AB - Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.
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U2 - 10.1126/science.1100533
DO - 10.1126/science.1100533
M3 - Article
C2 - 15375270
AN - SCOPUS:4544309783
SN - 0036-8075
VL - 305
SP - 1782
EP - 1786
JO - Science
JF - Science
IS - 5691
ER -