Two distinct actin networks drive the protrusion of migrating cells

A. Ponti, M. Machacek, S. L. Gupton, C. M. Waterman-Storer, G. Danuser

Research output: Contribution to journalArticle

534 Citations (Scopus)

Abstract

Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamellipodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.

Original languageEnglish (US)
Pages (from-to)1782-1786
Number of pages5
JournalScience
Volume305
Issue number5691
DOIs
StatePublished - Sep 17 2004

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Actomyosin
Pseudopodia
Motion Pictures
Cytoskeleton
Actin Cytoskeleton
Cell Movement
Actins
Microscopy
Epithelial Cells

ASJC Scopus subject areas

  • General

Cite this

Ponti, A., Machacek, M., Gupton, S. L., Waterman-Storer, C. M., & Danuser, G. (2004). Two distinct actin networks drive the protrusion of migrating cells. Science, 305(5691), 1782-1786. https://doi.org/10.1126/science.1100533

Two distinct actin networks drive the protrusion of migrating cells. / Ponti, A.; Machacek, M.; Gupton, S. L.; Waterman-Storer, C. M.; Danuser, G.

In: Science, Vol. 305, No. 5691, 17.09.2004, p. 1782-1786.

Research output: Contribution to journalArticle

Ponti, A, Machacek, M, Gupton, SL, Waterman-Storer, CM & Danuser, G 2004, 'Two distinct actin networks drive the protrusion of migrating cells', Science, vol. 305, no. 5691, pp. 1782-1786. https://doi.org/10.1126/science.1100533
Ponti A, Machacek M, Gupton SL, Waterman-Storer CM, Danuser G. Two distinct actin networks drive the protrusion of migrating cells. Science. 2004 Sep 17;305(5691):1782-1786. https://doi.org/10.1126/science.1100533
Ponti, A. ; Machacek, M. ; Gupton, S. L. ; Waterman-Storer, C. M. ; Danuser, G. / Two distinct actin networks drive the protrusion of migrating cells. In: Science. 2004 ; Vol. 305, No. 5691. pp. 1782-1786.
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