Two distinct types of endothelin receptors are present on chick cardiac membranes

Hirotoshi Watanabe, Hitoshi Miyazaki, Motohiro Kondoh, Yasushi Masuda, Sadao Kimura, Masashi Yanagisawa, Tomoh Masaki, Kazuo Murakami

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Competitive displacement experiments of 125I-endothelin (ET)-1, -2, or -3 binding to chick cardiac membranes were performed with unlabeled ET-1, -2, -3, and sarafotoxin S6b (STX) as competitors. 125I-ET-1 and -2 binding was competitively inhibited by increasing concentrations of these unlabeled peptides in the same order; i.e. ET-2 ≥ ET-1 > ET-3 > STX. In contrast, the order of potency in displacing 125I-ET-3 binding was ET-3 > ET-2 ≥ ET-1 > STX. Affinity labeling of the membranes by cross-linking with 125I-ET-1 and -2 via disuccinimidyl tartarate yielded one major specific band with an apparent Mr=53,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography. On the other hand, affinity labeling with 125I-ET-3 showed that two major and one minor bands of Mr=34,000, 46,000, and 53,000, respectively, were specifically labeled. These results indicate the presence of two distinct types of ET receptors, one of which has higher affinity for ET-1 and -2 than ET-3 and the other is conversely ET-3-preferring.

Original languageEnglish (US)
Pages (from-to)1252-1259
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number3
DOIs
StatePublished - Jun 30 1989

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Endothelin-2
Endothelin Receptors
Endothelin-3
Endothelin-1
Membranes
Labeling
Electrophoresis
Autoradiography
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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Two distinct types of endothelin receptors are present on chick cardiac membranes. / Watanabe, Hirotoshi; Miyazaki, Hitoshi; Kondoh, Motohiro; Masuda, Yasushi; Kimura, Sadao; Yanagisawa, Masashi; Masaki, Tomoh; Murakami, Kazuo.

In: Biochemical and Biophysical Research Communications, Vol. 161, No. 3, 30.06.1989, p. 1252-1259.

Research output: Contribution to journalArticle

Watanabe, Hirotoshi ; Miyazaki, Hitoshi ; Kondoh, Motohiro ; Masuda, Yasushi ; Kimura, Sadao ; Yanagisawa, Masashi ; Masaki, Tomoh ; Murakami, Kazuo. / Two distinct types of endothelin receptors are present on chick cardiac membranes. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 161, No. 3. pp. 1252-1259.
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