Two independent pathways of helper activity provided by a single T cell clone

M. Shigeta, S. Takahara, S. J. Knox, T. Ishihara, E. S. Vitetta, C. G. Fathman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Data presented in this paper demonstrate the existence of two separate pathways by which a single T cell clone can induce B cell differentiation. With the use of high doses of antigen, a T cell clone can induce a primary antibody response in unprimed B cells. With the use of low doses of antigen, the same T cell clone can induce an immunoglobulin (Ig)G response in primed B cells. The primary response is accompanied by T cell proliferation and lymphokine production (interleukin 2, B cell growth factor, B cell differentiation factor for immunoglobulin M, and B cell differentiation factor for immunoglobulin G). The secondary response does not require proliferation and occurs independently of detectable lymphokine production. Variants of the wild type T cell helper clone have been isolated. One variant can provide help to unprimed B cells when high doses of antigen are used. This variant cannot provide help to primed B cells when low doses of antigen are used, nor can it provide help to CBA/N 'xid' B cells at any antigen concentration tested. Additional variants have been isolated that proliferate on antigen-pulsed-presenting cells, but fail to secrete detectable lymphokines and do not induce B cell differentiation. These results suggest that a single T cell helper clone has multiple functional activities that can be independently expressed.

Original languageEnglish (US)
Pages (from-to)34-38
Number of pages5
JournalJournal of Immunology
Volume136
Issue number1
StatePublished - 1986

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B-Lymphocytes
Clone Cells
T-Lymphocytes
Lymphokines
Antigens
Helper-Inducer T-Lymphocytes
Cell Differentiation
Interleukin-6
Immunoglobulin G
Antigen-Presenting Cells
Antibody Formation
Interleukin-2
Immunoglobulin M
Intercellular Signaling Peptides and Proteins
Cell Proliferation

ASJC Scopus subject areas

  • Immunology

Cite this

Shigeta, M., Takahara, S., Knox, S. J., Ishihara, T., Vitetta, E. S., & Fathman, C. G. (1986). Two independent pathways of helper activity provided by a single T cell clone. Journal of Immunology, 136(1), 34-38.

Two independent pathways of helper activity provided by a single T cell clone. / Shigeta, M.; Takahara, S.; Knox, S. J.; Ishihara, T.; Vitetta, E. S.; Fathman, C. G.

In: Journal of Immunology, Vol. 136, No. 1, 1986, p. 34-38.

Research output: Contribution to journalArticle

Shigeta, M, Takahara, S, Knox, SJ, Ishihara, T, Vitetta, ES & Fathman, CG 1986, 'Two independent pathways of helper activity provided by a single T cell clone', Journal of Immunology, vol. 136, no. 1, pp. 34-38.
Shigeta M, Takahara S, Knox SJ, Ishihara T, Vitetta ES, Fathman CG. Two independent pathways of helper activity provided by a single T cell clone. Journal of Immunology. 1986;136(1):34-38.
Shigeta, M. ; Takahara, S. ; Knox, S. J. ; Ishihara, T. ; Vitetta, E. S. ; Fathman, C. G. / Two independent pathways of helper activity provided by a single T cell clone. In: Journal of Immunology. 1986 ; Vol. 136, No. 1. pp. 34-38.
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