Two novel engineered bacteria for secretory expression of glutaryl 7-amino-cephalosporanic acid acylase

Yong Gang Zheng, Yong Li, Jian Feng Chen, Wei Hong Jiang, Guo Ping Zhao, En Duo Wang

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Two novel engineered bacteria, BL21(DE3)/pETCA1S and TG1/pSuperCA1S, were obtained which can secretory express the gene encoding glutaryl 7-amino-cephalosporanic acid acylase (GL-7ACA acylase) from Pseudomonas sp. 130 with high activity. The growth conditions of transformants for overproduction of GL-7ACA acylase were optimized: in intact cells of BL21(DE3)/pETCA1S and TG1/pSuperCA1S the activity of GL-7ACA acylase was 415 and 600 units g-1 dry cells, respectively. The highest specific activity of GL-7-ACA acylase is in the intact cell as compared with that of transformants constructed in our laboratory. In fiftieth generation of mutants transferred on agar plates the specific activity of GL-7ACA acylase remained constant.

Original languageEnglish (US)
Pages (from-to)1781-1787
Number of pages7
JournalBiotechnology Letters
Volume23
Issue number21
DOIs
StatePublished - Dec 1 2001
Externally publishedYes

Keywords

  • Cloramphenicol
  • Glutaryl 7-amino-cephalosporanic acid acylase
  • Kanamycin
  • Secretory expression

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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