Two-photon dyes and nucleic acid detection

Frédéric Bolze, Jacques Lux, Eduardo Peña, Manfred Heinlein, Man Shing Wong, Jean François Nicoud

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Two-photon excited microscopy (TPEM) has evolved in the past few years as an important tool for revealing living processes in cells and organisms. Since fluorophores used in epi-fluorescence or confocal microscopy have limited sensitivity in TPEM in biological media, chemists and biologists attempt to design new fluorochromes for this application. Here the engineering of dyes for the specific detection of DNA and RNA molecules is a great challenge. We describe here the conception, preparation, and characterization of new two-photon-sensitive fluorochromes that are derived from cyanine and malachite green dyes and are specific for the detection of DNA and RNA respectively.

Original languageEnglish (US)
Pages (from-to)29-39
Number of pages11
JournalNonlinear Optics Quantum Optics
Volume45
Issue number1-2
StatePublished - Dec 1 2012

Fingerprint

Nucleic acids
nucleic acids
Nucleic Acids
Coloring Agents
Photons
Dyes
dyes
microscopy
RNA
Fluorescent Dyes
acids
Microscopic examination
photons
DNA
deoxyribonucleic acid
Fluorophores
Confocal microscopy
Fluorescence microscopy
organisms
engineering

Keywords

  • Aptamer
  • DNA detection
  • Nucleic acid
  • RNA detection
  • Two-photon absorption
  • Two-photon microscopy

ASJC Scopus subject areas

  • Statistical and Nonlinear Physics
  • Electronic, Optical and Magnetic Materials
  • Computer Science(all)
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

Cite this

Bolze, F., Lux, J., Peña, E., Heinlein, M., Wong, M. S., & Nicoud, J. F. (2012). Two-photon dyes and nucleic acid detection. Nonlinear Optics Quantum Optics, 45(1-2), 29-39.

Two-photon dyes and nucleic acid detection. / Bolze, Frédéric; Lux, Jacques; Peña, Eduardo; Heinlein, Manfred; Wong, Man Shing; Nicoud, Jean François.

In: Nonlinear Optics Quantum Optics, Vol. 45, No. 1-2, 01.12.2012, p. 29-39.

Research output: Contribution to journalArticle

Bolze, F, Lux, J, Peña, E, Heinlein, M, Wong, MS & Nicoud, JF 2012, 'Two-photon dyes and nucleic acid detection', Nonlinear Optics Quantum Optics, vol. 45, no. 1-2, pp. 29-39.
Bolze F, Lux J, Peña E, Heinlein M, Wong MS, Nicoud JF. Two-photon dyes and nucleic acid detection. Nonlinear Optics Quantum Optics. 2012 Dec 1;45(1-2):29-39.
Bolze, Frédéric ; Lux, Jacques ; Peña, Eduardo ; Heinlein, Manfred ; Wong, Man Shing ; Nicoud, Jean François. / Two-photon dyes and nucleic acid detection. In: Nonlinear Optics Quantum Optics. 2012 ; Vol. 45, No. 1-2. pp. 29-39.
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