Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

Mikhail Y. Berezin; Chun Zhan; Hyeran Lee; Chulmin Joo; Walter J. Akers; Siavash Yazdanfar; Samuel Achilefu

doi:10.1021/jp207618e

Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

Mikhail Y. Berezin, Chun Zhan, Hyeran Lee, Chulmin Joo, Walter J. Akers, Siavash Yazdanfar, Samuel Achilefu

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.

Original language	English (US)
Pages (from-to)	11530-11535
Number of pages	6
Journal	Journal of Physical Chemistry B
Volume	115
Issue number	39
DOIs	https://doi.org/10.1021/jp207618e
State	Published - Oct 6 2011
Externally published	Yes

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Surfaces, Coatings and Films
Materials Chemistry

Access to Document

10.1021/jp207618e

Cite this

@article{be216fb3cc0645eda9a8b6ea23c1b09e,

title = "Two-photon optical properties of near-infrared dyes at 1.55 μm excitation",

abstract = "Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.",

author = "Berezin, {Mikhail Y.} and Chun Zhan and Hyeran Lee and Chulmin Joo and Akers, {Walter J.} and Siavash Yazdanfar and Samuel Achilefu",

year = "2011",

month = oct,

day = "6",

doi = "10.1021/jp207618e",

language = "English (US)",

volume = "115",

pages = "11530--11535",

journal = "Journal of Physical Chemistry B",

issn = "1520-6106",

publisher = "American Chemical Society",

number = "39",

}

TY - JOUR

T1 - Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

AU - Berezin, Mikhail Y.

AU - Zhan, Chun

AU - Lee, Hyeran

AU - Joo, Chulmin

AU - Akers, Walter J.

AU - Yazdanfar, Siavash

AU - Achilefu, Samuel

PY - 2011/10/6

Y1 - 2011/10/6

N2 - Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.

AB - Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.

UR - http://www.scopus.com/inward/record.url?scp=80053430091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053430091&partnerID=8YFLogxK

U2 - 10.1021/jp207618e

DO - 10.1021/jp207618e

M3 - Article

C2 - 21866928

AN - SCOPUS:80053430091

SN - 1520-6106

VL - 115

SP - 11530

EP - 11535

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

IS - 39

ER -

Two-photon optical properties of near-infrared dyes at 1.55 μm excitation

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this