Two rat homologues of Drosophila achaete-scute specifically expressed in neuronal precursors

Jane E. Johnson, Susan J. Birren, David J. Anderson

Research output: Contribution to journalArticle

430 Citations (Scopus)

Abstract

IN vertebrates, the peripheral nervous system is embryologically derived from the neural crest1. Although the earliest neural crest cells seem to be multipotent2,3, the molecular mechanisms responsible for the restriction of these cells to different sublineages4 are not understood. We therefore searched for developmental control genes expressed in crest cells or their derivatives. One important class of regulatory molecules comprises proteins with common DNA-binding and dimerization domains, the basic helix-loop-helix (B-HLH) region5. Members of this family include MyoD (ref. 6), a mammalian myogenic determination molecule, and proteins encoded by genes of the achaete-scute complex7-9 of Drosophila, which have an important role in neuronal determination10. From a sympathetic neuronal precursor cell line derived from the neural crest11 we have now isolated two different mammalian genes that are homologous to genes of the achaete-scute complex. The sequence of the B-HLH-encoding region of these genes is more similar to that of the genes of the achaete-scute complex than it is to that of other, mammalian members of the B-HLH family. At least one of these genes is transiently expressed in the embryonic rat nervous system, is not detected in non-neuronal tissues or cell lines, and is induced by nerve growth factor in PC12 cells.

Original languageEnglish (US)
Pages (from-to)858-861
Number of pages4
JournalNature
Volume346
Issue number6287
StatePublished - Aug 30 1990

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Achaete-Scute Complex Genome Region
Drosophila
Helix-Loop-Helix Motifs
Genes
Developmental Genes
Cell Line
Neural Crest
PC12 Cells
Peripheral Nervous System
Dimerization
Nerve Growth Factor
Nervous System
Vertebrates
Proteins
DNA

ASJC Scopus subject areas

  • General

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Two rat homologues of Drosophila achaete-scute specifically expressed in neuronal precursors. / Johnson, Jane E.; Birren, Susan J.; Anderson, David J.

In: Nature, Vol. 346, No. 6287, 30.08.1990, p. 858-861.

Research output: Contribution to journalArticle

Johnson, Jane E. ; Birren, Susan J. ; Anderson, David J. / Two rat homologues of Drosophila achaete-scute specifically expressed in neuronal precursors. In: Nature. 1990 ; Vol. 346, No. 6287. pp. 858-861.
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