Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein

Marjatta Uotila, Erkki Ruoslahti, Eva Engvall

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Monoclonal antibodies reacting with different antigenic determinants on the alpha-fetoprotein (AFP) molecule were used to develop a simplified 'sandwich' ELISA (enzyme-linked immunospecific assay) in which one monoclonal antibody was immobilized and another labeled with an enzyme. Due to their different specificities the antibody attached to the solid phase and the one conjugated to the enzyme did not compete for binding to AFP making it possible to complete the two antigen-antibody reactions required in a single, short incubation. The rapidity, simplicity and excellent sensitivity of this assay combined with the exquisite specificity associated with the use of two monoclonal antibodies make us believe that assays based on this principle will be useful in the quantitation of substances of medical and biological importance.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalJournal of Immunological Methods
Volume42
Issue number1
DOIs
StatePublished - Apr 16 1981

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Immunoenzyme Techniques
Monoclonal Antibodies
alpha-Fetoproteins
Enzymes
Antigen-Antibody Reactions
Antibody Specificity
Epitopes
human AFP protein

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. / Uotila, Marjatta; Ruoslahti, Erkki; Engvall, Eva.

In: Journal of Immunological Methods, Vol. 42, No. 1, 16.04.1981, p. 11-15.

Research output: Contribution to journalArticle

Uotila, Marjatta ; Ruoslahti, Erkki ; Engvall, Eva. / Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. In: Journal of Immunological Methods. 1981 ; Vol. 42, No. 1. pp. 11-15.
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