Tyrosine phosphorylation of scavenger receptor cysteine-rich WC1 is required for the WC1-mediated potentiation of TCR-induced T-cell proliferation

Fei Wang, Carolyn Herzig, Dar Ozer, Cynthia L. Baldwin, Janice C. Telfer

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Workshop cluster 1 (WC1) molecules are transmembrane glycoproteins uniquely expressed by γδ T cells. They belong to the scavenger receptor cysteine-rich superfamily and are encoded by a multi-gene family, which is divided on the basis of antibody reactivity, into three groups, WC1.1, WC1.2, and WC1.3. The potential role of WC1 as a co-stimulatory molecule for the γδ TCR is suggested by the presence of several tyrosine-based motifs in their intracellular domains. In this study, we found that WC1 was constitutively phosphorylated in ex vivo bovine γδ T cells and associated with src family tyrosine kinases. Crosslinking of WC1 molecules resulted in an increase in WC1 phosphorylation and co-crosslinking of WC1 and γδ TCR together prolonged WC1 phosphorylation. We identified the second tyrosine residue as the primary phosphorylation target in WC1.1 and WC1.2 intracellular sequences in both in vitro and in vivo assays. The cytoplasmic tails of WC1.1 and WC1.2 were phosphorylated on serine and PKC activity was required for PMA-induced endocytosis of WC1.1 or WC1.2. We found that phosphorylation of the second tyrosine in the WC1 cytoplasmic domain was required for the WC1-mediated potentiation of TCR-induced T-cell proliferation, suggesting that WC1 acts as a co-stimulatory molecule for γδ TCR.

Original languageEnglish (US)
Pages (from-to)254-266
Number of pages13
JournalEuropean Journal of Immunology
Volume39
Issue number1
DOIs
StatePublished - Mar 9 2009

Fingerprint

Scavenger Receptors
Cysteine
Tyrosine
Phosphorylation
Cell Proliferation
T-Lymphocytes
Education
src-Family Kinases
Endocytosis
Serine
Glycoproteins

Keywords

  • γδ T cells
  • Bovine
  • Cell surface molecules
  • Signal transduction

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Tyrosine phosphorylation of scavenger receptor cysteine-rich WC1 is required for the WC1-mediated potentiation of TCR-induced T-cell proliferation. / Wang, Fei; Herzig, Carolyn; Ozer, Dar; Baldwin, Cynthia L.; Telfer, Janice C.

In: European Journal of Immunology, Vol. 39, No. 1, 09.03.2009, p. 254-266.

Research output: Contribution to journalArticle

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