TY - JOUR
T1 - UbcD1, a Drosophila ubiquitin-conjugating enzyme required for proper telomere behavior
AU - Cenci, Giovanni
AU - Rawson, Robert B.
AU - Belloni, Giorgio
AU - Castrillon, Diego H.
AU - Tudor, Mark
AU - Petrucci, Romano
AU - Goldberg, Michael L.
AU - Wasserman, Steven A.
AU - Gatti, Maurizio
PY - 1997/4/1
Y1 - 1997/4/1
N2 - The end-to-end association of chromosomes through their telomeres has been observed in normal cells of certain organisms, as well as in senescent and tumor cells. The molecular mechanisms underlying this phenomenon are currently unknown. We show here that five independent mutant alleles in the Drosophila UbcD1 gene cause frequent telomere-telomere attachments during both mitosis and male meiosis that are not seen in wild type. These telomeric associations involve all the telomeres of the D. melanogaster chromosome complement, albeit with different frequencies. The pattern of telomeric associations observed in UbcD1 mutants suggests strongly that the interphase chromosomes of wild-type larval brain cells maintain a Rabl orientation within the nucleus, with the telomeres and centromeres segregated to opposite sides of the nucleus. The UbcD1 gene encodes a class I ubiquitin-conjugating (E2) enzyme. This indicates that ubiquitin-mediated proteolysis is normally needed to ensure proper telomere behavior during Drosophila cell division. We therefore suggest that at least one of the targets of UbcD1 ubiquitination is a telomere-associated polypeptide that may help maintain proper chromosomal orientation during interphase.
AB - The end-to-end association of chromosomes through their telomeres has been observed in normal cells of certain organisms, as well as in senescent and tumor cells. The molecular mechanisms underlying this phenomenon are currently unknown. We show here that five independent mutant alleles in the Drosophila UbcD1 gene cause frequent telomere-telomere attachments during both mitosis and male meiosis that are not seen in wild type. These telomeric associations involve all the telomeres of the D. melanogaster chromosome complement, albeit with different frequencies. The pattern of telomeric associations observed in UbcD1 mutants suggests strongly that the interphase chromosomes of wild-type larval brain cells maintain a Rabl orientation within the nucleus, with the telomeres and centromeres segregated to opposite sides of the nucleus. The UbcD1 gene encodes a class I ubiquitin-conjugating (E2) enzyme. This indicates that ubiquitin-mediated proteolysis is normally needed to ensure proper telomere behavior during Drosophila cell division. We therefore suggest that at least one of the targets of UbcD1 ubiquitination is a telomere-associated polypeptide that may help maintain proper chromosomal orientation during interphase.
KW - Drosophila melanogaster
KW - telomere behavior
KW - ubiquitin-conjugating enzymes
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U2 - 10.1101/gad.11.7.863
DO - 10.1101/gad.11.7.863
M3 - Article
C2 - 9106658
AN - SCOPUS:0030907252
SN - 0890-9369
VL - 11
SP - 863
EP - 875
JO - Genes and Development
JF - Genes and Development
IS - 7
ER -