Ubiquitination regulates the internalization, endolysosomal sorting, and signaling of the erythropoietin receptor

Gamze Betul Bulut, Rita Sulahian, Yue Ma, Nai Wen Chi, Lily Jun Shen Huang

Research output: Contribution to journalArticle

19 Scopus citations


Ubiquitination is a common mechanism of down-regulation of mitogenic receptors. Here, we show that ubiquitination of the erythropoietin receptor (EpoR) at Lys256 is necessary and sufficient for efficient Epo-induced receptor internalization, whereas ubiquitination at Lys 428 promotes trafficking of activated receptors to the lysosomes for degradation. Interestingly, EpoR that cannot be ubiquitinated has reduced mitogenic activities and ability to stimulate the STAT5, Ras/ MAPK, and PI3K/AKT signaling pathways. We therefore propose that ubiquitination of the EpoR critically controls both receptor down-regulation and downstream signaling.

Original languageEnglish (US)
Pages (from-to)6449-6457
Number of pages9
JournalJournal of Biological Chemistry
Issue number8
StatePublished - Feb 25 2011


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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