Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase

Chunguang Guo, Xiaodong Zhang, Stephen P. Fink, Petra Platzer, Keith Wilson, James K V Willson, Zhenghe Wang, Sanford D. Markowitz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Expression microarrays identified a novel transcript, designated as Ugene, whose expression is absent in normal colon and colon adenomas, but that is commonly induced in malignant colon cancers. These findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon cancer cases. In addition, Ugene expression was found to be elevated in many other common cancer types, including breast, lung, uterus, and ovary. Immunofluorescence of V5-tagged Ugene revealed it to have a nuclear localization. In a pull-down assay, uracil DNA glycosylase 2 (UNG2), an important enzyme in the base excision repair (BER) pathway, was identified as a partner protein that binds to Ugene. Coimmunoprecipitation and Western blot analysis confirmed the binding between the endogenous Ugene and UNG2 proteins. Using deletion constructs, we find that Ugene binds to the first 25 amino acids of the UNG2 NH2 terminus. We suggest that Ugene induction in cancer may contribute to the cancer phenotype by interacting with the BER pathway.

Original languageEnglish (US)
Pages (from-to)6118-6126
Number of pages9
JournalCancer Research
Volume68
Issue number15
DOIs
StatePublished - Aug 1 2008

Fingerprint

Uracil-DNA Glycosidase
Uracil
DNA Repair
Colonic Neoplasms
DNA
Colon
Neoplasms
Proteins
Northern Blotting
Adenoma
Uterus
Fluorescent Antibody Technique
Real-Time Polymerase Chain Reaction
Ovary
Western Blotting
Breast Neoplasms
Phenotype
Amino Acids
Lung
Enzymes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Guo, C., Zhang, X., Fink, S. P., Platzer, P., Wilson, K., Willson, J. K. V., ... Markowitz, S. D. (2008). Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase. Cancer Research, 68(15), 6118-6126. https://doi.org/10.1158/0008-5472.CAN-08-1259

Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase. / Guo, Chunguang; Zhang, Xiaodong; Fink, Stephen P.; Platzer, Petra; Wilson, Keith; Willson, James K V; Wang, Zhenghe; Markowitz, Sanford D.

In: Cancer Research, Vol. 68, No. 15, 01.08.2008, p. 6118-6126.

Research output: Contribution to journalArticle

Guo, C, Zhang, X, Fink, SP, Platzer, P, Wilson, K, Willson, JKV, Wang, Z & Markowitz, SD 2008, 'Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase', Cancer Research, vol. 68, no. 15, pp. 6118-6126. https://doi.org/10.1158/0008-5472.CAN-08-1259
Guo, Chunguang ; Zhang, Xiaodong ; Fink, Stephen P. ; Platzer, Petra ; Wilson, Keith ; Willson, James K V ; Wang, Zhenghe ; Markowitz, Sanford D. / Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase. In: Cancer Research. 2008 ; Vol. 68, No. 15. pp. 6118-6126.
@article{49ea5bcc719147fd8f1a22269ef5d055,
title = "Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase",
abstract = "Expression microarrays identified a novel transcript, designated as Ugene, whose expression is absent in normal colon and colon adenomas, but that is commonly induced in malignant colon cancers. These findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon cancer cases. In addition, Ugene expression was found to be elevated in many other common cancer types, including breast, lung, uterus, and ovary. Immunofluorescence of V5-tagged Ugene revealed it to have a nuclear localization. In a pull-down assay, uracil DNA glycosylase 2 (UNG2), an important enzyme in the base excision repair (BER) pathway, was identified as a partner protein that binds to Ugene. Coimmunoprecipitation and Western blot analysis confirmed the binding between the endogenous Ugene and UNG2 proteins. Using deletion constructs, we find that Ugene binds to the first 25 amino acids of the UNG2 NH2 terminus. We suggest that Ugene induction in cancer may contribute to the cancer phenotype by interacting with the BER pathway.",
author = "Chunguang Guo and Xiaodong Zhang and Fink, {Stephen P.} and Petra Platzer and Keith Wilson and Willson, {James K V} and Zhenghe Wang and Markowitz, {Sanford D.}",
year = "2008",
month = "8",
day = "1",
doi = "10.1158/0008-5472.CAN-08-1259",
language = "English (US)",
volume = "68",
pages = "6118--6126",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "15",

}

TY - JOUR

T1 - Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase

AU - Guo, Chunguang

AU - Zhang, Xiaodong

AU - Fink, Stephen P.

AU - Platzer, Petra

AU - Wilson, Keith

AU - Willson, James K V

AU - Wang, Zhenghe

AU - Markowitz, Sanford D.

PY - 2008/8/1

Y1 - 2008/8/1

N2 - Expression microarrays identified a novel transcript, designated as Ugene, whose expression is absent in normal colon and colon adenomas, but that is commonly induced in malignant colon cancers. These findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon cancer cases. In addition, Ugene expression was found to be elevated in many other common cancer types, including breast, lung, uterus, and ovary. Immunofluorescence of V5-tagged Ugene revealed it to have a nuclear localization. In a pull-down assay, uracil DNA glycosylase 2 (UNG2), an important enzyme in the base excision repair (BER) pathway, was identified as a partner protein that binds to Ugene. Coimmunoprecipitation and Western blot analysis confirmed the binding between the endogenous Ugene and UNG2 proteins. Using deletion constructs, we find that Ugene binds to the first 25 amino acids of the UNG2 NH2 terminus. We suggest that Ugene induction in cancer may contribute to the cancer phenotype by interacting with the BER pathway.

AB - Expression microarrays identified a novel transcript, designated as Ugene, whose expression is absent in normal colon and colon adenomas, but that is commonly induced in malignant colon cancers. These findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon cancer cases. In addition, Ugene expression was found to be elevated in many other common cancer types, including breast, lung, uterus, and ovary. Immunofluorescence of V5-tagged Ugene revealed it to have a nuclear localization. In a pull-down assay, uracil DNA glycosylase 2 (UNG2), an important enzyme in the base excision repair (BER) pathway, was identified as a partner protein that binds to Ugene. Coimmunoprecipitation and Western blot analysis confirmed the binding between the endogenous Ugene and UNG2 proteins. Using deletion constructs, we find that Ugene binds to the first 25 amino acids of the UNG2 NH2 terminus. We suggest that Ugene induction in cancer may contribute to the cancer phenotype by interacting with the BER pathway.

UR - http://www.scopus.com/inward/record.url?scp=51049100628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51049100628&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-08-1259

DO - 10.1158/0008-5472.CAN-08-1259

M3 - Article

C2 - 18676834

AN - SCOPUS:51049100628

VL - 68

SP - 6118

EP - 6126

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 15

ER -