Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase

Chunguang Guo, Xiaodong Zhang, Stephen P. Fink, Petra Platzer, Keith Wilson, James K V Willson, Zhenghe Wang, Sanford D. Markowitz

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13 Scopus citations

Abstract

Expression microarrays identified a novel transcript, designated as Ugene, whose expression is absent in normal colon and colon adenomas, but that is commonly induced in malignant colon cancers. These findings were validated by real-time PCR and Northern blot analysis in an independent panel of colon cancer cases. In addition, Ugene expression was found to be elevated in many other common cancer types, including breast, lung, uterus, and ovary. Immunofluorescence of V5-tagged Ugene revealed it to have a nuclear localization. In a pull-down assay, uracil DNA glycosylase 2 (UNG2), an important enzyme in the base excision repair (BER) pathway, was identified as a partner protein that binds to Ugene. Coimmunoprecipitation and Western blot analysis confirmed the binding between the endogenous Ugene and UNG2 proteins. Using deletion constructs, we find that Ugene binds to the first 25 amino acids of the UNG2 NH2 terminus. We suggest that Ugene induction in cancer may contribute to the cancer phenotype by interacting with the BER pathway.

Original languageEnglish (US)
Pages (from-to)6118-6126
Number of pages9
JournalCancer Research
Volume68
Issue number15
DOIs
Publication statusPublished - Aug 1 2008

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Guo, C., Zhang, X., Fink, S. P., Platzer, P., Wilson, K., Willson, J. K. V., ... Markowitz, S. D. (2008). Ugene, a newly identified protein that is commonly overexpressed in cancer and binds uracil DNA gycosylase. Cancer Research, 68(15), 6118-6126. https://doi.org/10.1158/0008-5472.CAN-08-1259