Ultrasonic assisted enzymatic digestion (USAED) coupled with high performance liquid chromatography and electrothermal atomic absorption spectrometry as a powerful tool for total selenium and selenium species control in Se-enriched food supplements

G. Vale, A. Rodrigues, A. Rocha, R. Rial, A. M. Mota, M. L. Gonçalves, L. P. Fonseca, J. L. Capelo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The suitability of hyphenated USAED with HPLC separation and ET-AAS determination as a new rapid methodology for Se control in Se-enriched food supplements is demonstrated. Total Se determination and Se speciation are accomplished in a single sample treatment using low sample amounts (ca. 10 mg), and low extracting volume (1 mL). The total Se content in seven of the 10 Se-enriched supplements studied was in agreement with the values obtained after microwave pressurized acid digestion, MW, (test t, p = 0.05). The Se species studied were Se(IV), Se(VI), SeMet, SeMeSeCys, and SeCys2, being some of the most common found in the 10 supplements studied. Although SeMet was the Se species expected to be present at the highest concentration in most Se-enriched food supplements, we detected it in only three of the 10 samples studied. In the other seven samples, two of them had Se(IV) as the main Se species. The other five supplements had Se species that did not match with any of the five standards selected by us. We have also systematically demonstrated that ultrasonication does not alter the following Se species: Se(IV), Se(VI), SeMet, SeMeSeCys, and SeCys2. The new procedure can be easily adapted to more Se species and can be routinely used for Se control in Se-enriched food supplements. Concerning the supplements studied, our results suggest that stricter control on the Se content in enriched food supplements in terms of Se species will need to become mandatory.

Original languageEnglish (US)
Pages (from-to)268-274
Number of pages7
JournalFood Chemistry
Volume121
Issue number1
DOIs
StatePublished - Jul 1 2010

Fingerprint

Fortified Food
enriched foods
Atomic absorption spectrometry
atomic absorption spectrometry
High performance liquid chromatography
Selenium
Dietary Supplements
Ultrasonics
selenium
dietary supplements
Digestion
Spectrum Analysis
ultrasonics
high performance liquid chromatography
digestion
High Pressure Liquid Chromatography
sampling
Microwaves
Acids
acids

Keywords

  • ET-AAS
  • HPLC
  • Se
  • Speciation
  • USAED

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Cite this

Ultrasonic assisted enzymatic digestion (USAED) coupled with high performance liquid chromatography and electrothermal atomic absorption spectrometry as a powerful tool for total selenium and selenium species control in Se-enriched food supplements. / Vale, G.; Rodrigues, A.; Rocha, A.; Rial, R.; Mota, A. M.; Gonçalves, M. L.; Fonseca, L. P.; Capelo, J. L.

In: Food Chemistry, Vol. 121, No. 1, 01.07.2010, p. 268-274.

Research output: Contribution to journalArticle

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abstract = "The suitability of hyphenated USAED with HPLC separation and ET-AAS determination as a new rapid methodology for Se control in Se-enriched food supplements is demonstrated. Total Se determination and Se speciation are accomplished in a single sample treatment using low sample amounts (ca. 10 mg), and low extracting volume (1 mL). The total Se content in seven of the 10 Se-enriched supplements studied was in agreement with the values obtained after microwave pressurized acid digestion, MW, (test t, p = 0.05). The Se species studied were Se(IV), Se(VI), SeMet, SeMeSeCys, and SeCys2, being some of the most common found in the 10 supplements studied. Although SeMet was the Se species expected to be present at the highest concentration in most Se-enriched food supplements, we detected it in only three of the 10 samples studied. In the other seven samples, two of them had Se(IV) as the main Se species. The other five supplements had Se species that did not match with any of the five standards selected by us. We have also systematically demonstrated that ultrasonication does not alter the following Se species: Se(IV), Se(VI), SeMet, SeMeSeCys, and SeCys2. The new procedure can be easily adapted to more Se species and can be routinely used for Se control in Se-enriched food supplements. Concerning the supplements studied, our results suggest that stricter control on the Se content in enriched food supplements in terms of Se species will need to become mandatory.",
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