Ultrastructural analysis of crystalloid endoplasmic reticulum in UT-1 cells and its disappearance in response to cholesterol

R. G W Anderson, L. Orci, M. S. Brown

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Abstract

The crystalloid endoplasmic reticulum (ER) consists of hexagonally packed membrane tubules that contain 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase), an intrinsic membrane protein that catalyses the rate-limiting step in cholesterol synthesis. The crystalloid ER appears in a clone of Chinese hamster ovary cells, designated UT-1, that contain high levels of HMG CoA reductase as a result of growth in the presence of compactin, a competitive inhibitor of the reductase. In the present studies, we have used ultrastructural morphometry to estimate that the crystalloid ER: occupies about 15% of the volume of UT-1 cells; contains 3.4-fold more membrane area than the plasma membrane; and contains less than 700 subunits of HMG CoA reductase per μm2 of membrane surface. The crystalloid ER tubules contain 2000 intramembrane particles per μm2 with a mean diameter of 10.4 nm, as determined by freeze-fracture. The crystalloid ER membranes are low in cholesterol, as indicated by the small number of filipin-cholesterol complexes in freeze-fracture images after treatment with filipin. The addition of cholesterol or related sterols to UT-1 cells promoted a rapid and stepwise disappearance of the crystalloid ER. Initially, the crystalloid ER fragmented into randomly arranged vesicles and tubules. Subsequently, membrane-bound structures disappeared from the cell so that after incubation with cholesterol for 24-72 h, the cells appeared completely normal. We found no morphological evidence that autophagic vacuoles participate in the degradation. We conclude: that the crystalloid ER is more extensive than necessary merely to support HMG CoA reductase; and that upon exposure to cholesterol the crystalloid ER is degraded by a process that does not involve autophagy.

Original languageEnglish (US)
Pages (from-to)1-20
Number of pages20
JournalJournal of Cell Science
VolumeVol. 63
StatePublished - 1983

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Endoplasmic Reticulum
Cholesterol
Oxidoreductases
Membranes
Filipin
crystalloid solutions
Autophagy
Sterols
Vacuoles
Cricetulus
Ovary
Membrane Proteins
Clone Cells
Cell Membrane
3-hydroxy-3-methylglutaryl-coenzyme A
Growth

ASJC Scopus subject areas

  • Cell Biology

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Ultrastructural analysis of crystalloid endoplasmic reticulum in UT-1 cells and its disappearance in response to cholesterol. / Anderson, R. G W; Orci, L.; Brown, M. S.

In: Journal of Cell Science, Vol. Vol. 63, 1983, p. 1-20.

Research output: Contribution to journalArticle

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