Ultrastructural analysis of myoblast fusion in Drosophila

Shiliang Zhang, Elizabeth Chen

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Myoblast fusion in Drosophila has become a powerful genetic system with which to unravel the mechanisms underlying cell fusion. The identification of important components of myoblast fusion by genetic analysis has led to a molecular pathway toward our understanding of this cellular process. In addition to the application of immunohistochemistry and live imaging techniques to visualize myoblast fusion at the light microscopic level, ultrastructural analysis using electron microscopy remains an indispensable tool to reveal fusion intermediates and specific membrane events at sites of fusion. In this chapter, we describe conventional chemical fixation and high-pressure freezing/freeze substitution methods for visualizing fusion intermediates during Drosophila myoblast fusion. Furthermore, we describe an immunoelectron microscopic method for localizing specific proteins relative to the fusion apparatus.

Original languageEnglish (US)
Title of host publicationCell Fusion
Subtitle of host publicationOverviews and Methods
PublisherHumana Press
Pages275-297
Number of pages23
Volume475
ISBN (Print)9781588299116
DOIs
StatePublished - Jan 1 2008

Publication series

NameMethods in Molecular Biology
Volume475
ISSN (Print)1064-3745

Keywords

  • Cell fusion
  • Chemical fixation
  • Drosophila
  • Electron microscopy
  • High-pressure freezing/freeze substitution
  • Immunoelectron microscopy
  • Myoblast fusion

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Zhang, S., & Chen, E. (2008). Ultrastructural analysis of myoblast fusion in Drosophila. In Cell Fusion: Overviews and Methods (Vol. 475, pp. 275-297). (Methods in Molecular Biology; Vol. 475). Humana Press. https://doi.org/10.1007/978-1-59745-250-2_16