Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF- expressing tumors and adenoviral vectors

Dian Feng, Janice A. Nagy, Rolf A. Brekken, Anna Pettersson, Eleanor J. Manseau, Kathryn Pyne, Richard Mulligan, Philip E. Thorpe, Harold F. Dvorak, Ann M. Dvorak

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) interacts with two high-affinity tyrosine kinase receptors, VEGFR- 1 and VEGFR-2, to increase microvascular permeability and induce angiogenesis. Both receptors are selectively expressed by vascular endothelial cells and are strikingly increased in tumor vessels. We used a specific antibody to localize VEGFR-2 (FLK-1, KDR) in microvascular endothelium of normal mouse kidneys and in the microvessels induced by the TA3/St mammary tumor or by infection with an adenoviral vector engineered to express VPF/VEGF. A pre-embedding method was employed at the light and electron microscopic levels using either nanogold or peroxidase as reporters. Equivalent staining was observed on both the luminal and abluminal surfaces of tumor- and adenovirus-induced vascular endothelium, but plasma membranes at interendothelial junctions were spared except at sites connected to vesiculovacuolar organelles (VVOs). VEGFR-2 was also localized to the membranes and stomatal diaphragms of some VVOs. This staining distribution is consistent with a model in which VPF/VEGF increases microvascular permeability by opening VVOs to allow the transendothelial cell passage of plasma and plasma proteins.

Original languageEnglish (US)
Pages (from-to)545-555
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume48
Issue number4
StatePublished - Apr 2000

Fingerprint

Vascular Endothelial Growth Factor Receptor-2
Vascular Endothelial Growth Factor A
Kidney
Organelles
Neoplasms
Capillary Permeability
Staining and Labeling
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelium
Receptor Protein-Tyrosine Kinases
Microvessels
Diaphragm
Plasma Cells
Adenoviridae
Peroxidase
Endothelium
Blood Proteins
Endothelial Cells
Cell Membrane
Electrons

Keywords

  • (VVO)
  • Endothelial cells
  • Fetal liver kinase 1 (Flk-1)
  • Kinase insert domain- containing receptor (KDR)
  • Mouse kidney
  • Tumor vessels
  • Ultrastructure immunocytochemistry
  • Vascular endothelial growth factor (VEGF)
  • Vascular endothelial growth factor receptor (VEGFR)
  • Vascular permeability factor (VPF)
  • Vascular permeability factor receptor (VPFR)
  • Vesiculovacuolar organelle

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF- expressing tumors and adenoviral vectors. / Feng, Dian; Nagy, Janice A.; Brekken, Rolf A.; Pettersson, Anna; Manseau, Eleanor J.; Pyne, Kathryn; Mulligan, Richard; Thorpe, Philip E.; Dvorak, Harold F.; Dvorak, Ann M.

In: Journal of Histochemistry and Cytochemistry, Vol. 48, No. 4, 04.2000, p. 545-555.

Research output: Contribution to journalArticle

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abstract = "Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) interacts with two high-affinity tyrosine kinase receptors, VEGFR- 1 and VEGFR-2, to increase microvascular permeability and induce angiogenesis. Both receptors are selectively expressed by vascular endothelial cells and are strikingly increased in tumor vessels. We used a specific antibody to localize VEGFR-2 (FLK-1, KDR) in microvascular endothelium of normal mouse kidneys and in the microvessels induced by the TA3/St mammary tumor or by infection with an adenoviral vector engineered to express VPF/VEGF. A pre-embedding method was employed at the light and electron microscopic levels using either nanogold or peroxidase as reporters. Equivalent staining was observed on both the luminal and abluminal surfaces of tumor- and adenovirus-induced vascular endothelium, but plasma membranes at interendothelial junctions were spared except at sites connected to vesiculovacuolar organelles (VVOs). VEGFR-2 was also localized to the membranes and stomatal diaphragms of some VVOs. This staining distribution is consistent with a model in which VPF/VEGF increases microvascular permeability by opening VVOs to allow the transendothelial cell passage of plasma and plasma proteins.",
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T1 - Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF- expressing tumors and adenoviral vectors

AU - Feng, Dian

AU - Nagy, Janice A.

AU - Brekken, Rolf A.

AU - Pettersson, Anna

AU - Manseau, Eleanor J.

AU - Pyne, Kathryn

AU - Mulligan, Richard

AU - Thorpe, Philip E.

AU - Dvorak, Harold F.

AU - Dvorak, Ann M.

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AB - Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) interacts with two high-affinity tyrosine kinase receptors, VEGFR- 1 and VEGFR-2, to increase microvascular permeability and induce angiogenesis. Both receptors are selectively expressed by vascular endothelial cells and are strikingly increased in tumor vessels. We used a specific antibody to localize VEGFR-2 (FLK-1, KDR) in microvascular endothelium of normal mouse kidneys and in the microvessels induced by the TA3/St mammary tumor or by infection with an adenoviral vector engineered to express VPF/VEGF. A pre-embedding method was employed at the light and electron microscopic levels using either nanogold or peroxidase as reporters. Equivalent staining was observed on both the luminal and abluminal surfaces of tumor- and adenovirus-induced vascular endothelium, but plasma membranes at interendothelial junctions were spared except at sites connected to vesiculovacuolar organelles (VVOs). VEGFR-2 was also localized to the membranes and stomatal diaphragms of some VVOs. This staining distribution is consistent with a model in which VPF/VEGF increases microvascular permeability by opening VVOs to allow the transendothelial cell passage of plasma and plasma proteins.

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