Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma

Ashley Bui, Yongbin Choi, Arthur E. Frankel, Andrew Y. Koh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Gut microbiota influence and modulate host immune responses. In preclinical cancer models, mice lacking gut microbiota have a markedly diminished response to immune checkpoint inhibitor therapy. Further, in melanoma patients, specific commensal gut microbiota have been associated with a positive clinical response to immunotherapy. In order to study the gut microbiome and metabolome, we have developed methods for fecal sample collection and processing, microbiome and metabolome profiling, and bioinformatic analysis. This protocol will be a useful tool for interrogating the taxonomic composition and functional output of a melanoma patient’s gut microbiome.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages461-474
Number of pages14
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2265
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Fecal
  • Immunotherapy
  • Melanoma
  • Metabolomics
  • Microbiome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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