Uniform and scalable light-sheets generated by extended focusing

Kevin M. Dean, Reto Fiolka

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Light-sheet fluorescence microscopy (LSFM) affords highly parallelized 3D imaging with optical sectioning capability and minimal light exposure. However, using Gaussian beams for light-sheet generation results in a trade-off between beam waist thickness and the area over which the beam can approximate a light-sheet. Here, we present a novel form of LSFM that uses incoherent extended focusing to produce divergence free light-sheets with near diffraction-limited resolution and uniform intensity distribution along the propagation direction. We demonstrate the imaging performance of the new technique by volumetric imaging of beads, collagen fibers, and melanoma cancer cells with sub-cellular resolution.

Original languageEnglish (US)
Pages (from-to)26141-26152
Number of pages12
JournalOptics Express
Volume22
Issue number21
DOIs
StatePublished - Oct 20 2014

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microscopy
fluorescence
collagens
beads
divergence
cancer
fibers
propagation
diffraction

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Uniform and scalable light-sheets generated by extended focusing. / Dean, Kevin M.; Fiolka, Reto.

In: Optics Express, Vol. 22, No. 21, 20.10.2014, p. 26141-26152.

Research output: Contribution to journalArticle

Dean, Kevin M. ; Fiolka, Reto. / Uniform and scalable light-sheets generated by extended focusing. In: Optics Express. 2014 ; Vol. 22, No. 21. pp. 26141-26152.
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