Unitary cardiac Na+,Ca2+ exchange current magnitudes determined from channel-like noise and charge movements of ion transport

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Abstract

The cardiac Na+,Ca2+ exchanger (NCX1) is thought to achieve a high turnover rate, but all estimates to date are indirect. Two new strategies demonstrate that maximum unitary exchange currents are about 1 fA (6000 unitary charges per s) and that they fluctuate between on and off levels similar to ion channel currents. First, exchange current noise has been identified in small cardiac patches with properties expected for a gated transport process. Noise power density spectra correlate well with exchanger inactivation kinetics, and the noise has a predicted bell-shaped dependence on the activation state of the exchanger. From the magnitudes of exchange current noise, maximum unitary exchange currents are estimated to be 0.6-1.3 fA. Second, charge movements with rates of ~5000 s-1 have been isolated for the transport of both Na+ and Ca2+ in giant membrane patches using nonsaturating ion concentrations. The Na+ transport reactions are disabled or 'immobilized' by exchanger inactivation reactions, thus confirming that inactivation generates fully inactive exchanger states.

Original languageEnglish (US)
Pages (from-to)759-768
Number of pages10
JournalBiophysical Journal
Volume71
Issue number2
StatePublished - Aug 1996

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Ion Transport
Noise
Ion Channels
Ions
Membranes

ASJC Scopus subject areas

  • Biophysics

Cite this

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abstract = "The cardiac Na+,Ca2+ exchanger (NCX1) is thought to achieve a high turnover rate, but all estimates to date are indirect. Two new strategies demonstrate that maximum unitary exchange currents are about 1 fA (6000 unitary charges per s) and that they fluctuate between on and off levels similar to ion channel currents. First, exchange current noise has been identified in small cardiac patches with properties expected for a gated transport process. Noise power density spectra correlate well with exchanger inactivation kinetics, and the noise has a predicted bell-shaped dependence on the activation state of the exchanger. From the magnitudes of exchange current noise, maximum unitary exchange currents are estimated to be 0.6-1.3 fA. Second, charge movements with rates of ~5000 s-1 have been isolated for the transport of both Na+ and Ca2+ in giant membrane patches using nonsaturating ion concentrations. The Na+ transport reactions are disabled or 'immobilized' by exchanger inactivation reactions, thus confirming that inactivation generates fully inactive exchanger states.",
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