Universal light-sheet generation with field synthesis

Bo Jui Chang, Mark Kittisopikul, Kevin M. Dean, Philippe Roudot, Erik S. Welf, Reto Fiolka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.

Original languageEnglish (US)
Pages (from-to)235-238
Number of pages4
JournalNature methods
Volume16
Issue number3
DOIs
StatePublished - Mar 1 2019

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Light
Microscopy
Microscopic examination
Photobleaching
Lighting
Optical design
Throughput
Scanning

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Universal light-sheet generation with field synthesis. / Chang, Bo Jui; Kittisopikul, Mark; Dean, Kevin M.; Roudot, Philippe; Welf, Erik S.; Fiolka, Reto.

In: Nature methods, Vol. 16, No. 3, 01.03.2019, p. 235-238.

Research output: Contribution to journalArticle

Chang, BJ, Kittisopikul, M, Dean, KM, Roudot, P, Welf, ES & Fiolka, R 2019, 'Universal light-sheet generation with field synthesis', Nature methods, vol. 16, no. 3, pp. 235-238. https://doi.org/10.1038/s41592-019-0327-9
Chang, Bo Jui ; Kittisopikul, Mark ; Dean, Kevin M. ; Roudot, Philippe ; Welf, Erik S. ; Fiolka, Reto. / Universal light-sheet generation with field synthesis. In: Nature methods. 2019 ; Vol. 16, No. 3. pp. 235-238.
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