Abstract
We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.
Original language | English (US) |
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Pages (from-to) | 235-238 |
Number of pages | 4 |
Journal | Nature methods |
Volume | 16 |
Issue number | 3 |
DOIs | |
State | Published - Mar 1 2019 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology