Unraveling innate immunity using large scale N-ethyl-N-nitrosourea mutagenesis

Kasper Hoebe, B. Beutler

Research output: Contribution to journalReview article

19 Scopus citations

Abstract

With the mouse genome almost entirely sequenced and readily accessible to all who wish to examine it, the challenge across most biological disciplines now lies in the decipherment of gene and protein function rather than in the realm of gene identification per se. In the field of innate immunity, forward genetic methods have repeatedly been applied to identify key sensors, adapters, and effector molecules. However, most spontaneous mutations that affect innate immune function have been mapped and cloned, and the need for new monogenic phenotypes has been felt evermore keenly. N-Ethyl-N-nitrosourea (ENU) mutagenesis is an efficient tool for the creation of aberrant monogenic innate immune response phenotypes. In this review, we will discuss the potential of the forward genetic approach and ENU mutagenesis to identify new genes and new functions of known genes related to innate immunity.

Original languageEnglish (US)
Pages (from-to)395-401
Number of pages7
JournalTissue Antigens
Volume65
Issue number5
DOIs
StatePublished - May 1 2005

Keywords

  • CD36
  • ENU mutagenesis
  • Forward genetics
  • Innate immunity
  • Trif

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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