TY - JOUR
T1 - Unraveling the dynamics of ribosome translocation
AU - Chen, Jin
AU - Tsai, Albert
AU - O'Leary, Seán E.
AU - Petrov, Alexey
AU - Puglisi, Joseph D.
N1 - Funding Information:
Single-molecule research in the Puglisi group is funded by NIH grants GM51266 and GM099687 . We would like to thank all members of Puglisi laboratory for helpful discussions.
PY - 2012/12
Y1 - 2012/12
N2 - Translocation is one of the key events in translation, requiring large-scale conformational changes in the ribosome, movements of two transfer RNAs (tRNAs) across a distance of more than 20. Å, and the coupled movement of the messenger RNA (mRNA) by one codon, completing one cycle of peptide-chain elongation. Translocation is catalyzed by elongation factor G (EF-G in bacteria), which hydrolyzes GTP in the process. However, how the conformational rearrangements of the ribosome actually drive the movements of the tRNAs and how EF-G GTP hydrolysis plays a role in this process are still unclear. Fluorescence methods, both single-molecule and bulk, have provided a dynamic view of translocation, allowing us to follow the different conformational changes of the ribosome in real-time. The application of electron microscopy has revealed new conformational intermediates during translocation and important structural rearrangements in the ribosome that drive tRNA movement, while computational approaches have added quantitative views of the translational pathway. These recent advances shed light on the process of translocation, providing insight on how to resolve the different descriptions of translocation in the current literature.
AB - Translocation is one of the key events in translation, requiring large-scale conformational changes in the ribosome, movements of two transfer RNAs (tRNAs) across a distance of more than 20. Å, and the coupled movement of the messenger RNA (mRNA) by one codon, completing one cycle of peptide-chain elongation. Translocation is catalyzed by elongation factor G (EF-G in bacteria), which hydrolyzes GTP in the process. However, how the conformational rearrangements of the ribosome actually drive the movements of the tRNAs and how EF-G GTP hydrolysis plays a role in this process are still unclear. Fluorescence methods, both single-molecule and bulk, have provided a dynamic view of translocation, allowing us to follow the different conformational changes of the ribosome in real-time. The application of electron microscopy has revealed new conformational intermediates during translocation and important structural rearrangements in the ribosome that drive tRNA movement, while computational approaches have added quantitative views of the translational pathway. These recent advances shed light on the process of translocation, providing insight on how to resolve the different descriptions of translocation in the current literature.
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U2 - 10.1016/j.sbi.2012.09.004
DO - 10.1016/j.sbi.2012.09.004
M3 - Review article
C2 - 23142574
AN - SCOPUS:84870388825
SN - 0959-440X
VL - 22
SP - 804
EP - 814
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
IS - 6
ER -