Unrestrained caspase-dependent cell death caused by loss of Diap 1 function requires the Drosophila Apaf-1 homolog, Dark

Antony Rodriguez, Po Chen, Holt Oliver, John M. Abrams

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

In mammals and Drosophila, apoptotic caspases are under positive control via the CED-4/Apaf-1/Dark adaptors and negative control via IAPs (inhibitor of ns). However, the in vivo genetic relationship between these opposing regulators is not known. In this study, we demonstrate that a dark mutation reverses catastrophic defects seen in Diap1 mutants and rescues cells specified for Diap1regulated cell death in development and in response to genotoxic stress. We also find that dark function is required for hyperactivation of caspases which occurs in the absence of Diap1. Since the action of dark is epistatic to that of Diap1, these findings demonstrate that caspase-dependent cell death requires concurrent positive input through Apaf-1-like proteins together with disruption of IAP-caspase complexes.

Original languageEnglish (US)
Pages (from-to)2189-2197
Number of pages9
JournalEMBO Journal
Volume21
Issue number9
DOIs
StatePublished - May 1 2002

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Keywords

  • Apaf-1
  • Apoptosis
  • Drosophila
  • IAP
  • PCD

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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