TY - JOUR
T1 - Unusual susceptibility of methicillin-resistant Staphylococcus aureus to erythromycin, clindamycin, gentamicin, and tetracycline at 30°C but not at 35°C
AU - Welch, W. D.
AU - Southern, P. M.
PY - 1984
Y1 - 1984
N2 - Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen in hospital environments, and optimal detection of MRSA requires nonroutine methods in clinical microbiology laboratories. One such method is an incubation temperature of 30° C in contrast to the more commonly used temperature of 35°C. To determine the percentage of MRSA isolates that would be missed if only one temperature were used, we evaluated methicillin resistance and susceptibility of 2,397 S aureus isolates by agar dilution at 30 and 35°C. Of the clinical isolates, 93% showed matching MICs of methicillin at both temperatures. Another 6.8% (162) showed different MICs at 30 and 35°C, with 60 of the 162 isolates (2.5% of all isolates studied) being resistant to methicillin at 30°C but susceptible at 35° C (temperature-discrepant MRSA). MICs of other antimicrobial agents, measured at 35° C, revealed an unusual pattern of susceptibility of these temperature-discrepa nt MRSA isolates. In contrast to previously reported resistance of MRSA at 35° C to erythromycin, clindamycin, gentamicin, and tetracycline, the temperature- discrepant MRSA isolates were susceptible to these agents. This resistance pattern may be of value in identifying questionable MRSA isolates when only one incubation temperature is used.
AB - Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen in hospital environments, and optimal detection of MRSA requires nonroutine methods in clinical microbiology laboratories. One such method is an incubation temperature of 30° C in contrast to the more commonly used temperature of 35°C. To determine the percentage of MRSA isolates that would be missed if only one temperature were used, we evaluated methicillin resistance and susceptibility of 2,397 S aureus isolates by agar dilution at 30 and 35°C. Of the clinical isolates, 93% showed matching MICs of methicillin at both temperatures. Another 6.8% (162) showed different MICs at 30 and 35°C, with 60 of the 162 isolates (2.5% of all isolates studied) being resistant to methicillin at 30°C but susceptible at 35° C (temperature-discrepant MRSA). MICs of other antimicrobial agents, measured at 35° C, revealed an unusual pattern of susceptibility of these temperature-discrepa nt MRSA isolates. In contrast to previously reported resistance of MRSA at 35° C to erythromycin, clindamycin, gentamicin, and tetracycline, the temperature- discrepant MRSA isolates were susceptible to these agents. This resistance pattern may be of value in identifying questionable MRSA isolates when only one incubation temperature is used.
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U2 - 10.1128/jcm.19.6.831-833.1984
DO - 10.1128/jcm.19.6.831-833.1984
M3 - Article
C2 - 6565708
AN - SCOPUS:0021258539
SN - 0095-1137
VL - 19
SP - 831
EP - 833
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 6
ER -