Unveiling a hidden 31P signal coresonating with extracellular inorganic phosphate by outer-volume-suppression and localized 31P MRS in the human brain at 7T

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Abstract

Purpose: The study was undertaken to demonstrate that there is more than 1 component in the extracellular Pi 31P signal ( Piex) acquired from human head using nonlocalized 31P MRS. Methods: Outer-volume-suppression (OVS) saturation and 1D/2D 31P CSI were utilized to reveal the presence of an additional component in the Piex signal. Results: 67% of the head extracellular Pi signal was attenuated upon OVS saturation of the peripheral meningeal tissues, likely reflecting elimination of the Pi signal in the meningeal fluids (the blood and CSF). Localized 1D/2D CSI data provided further support for this assignment. Upon correction for the meningeal contribution, the extracellular Pi concentration was 0.51±0.07mM, whereas the intracellular Pi was 0.85±0.10mM. The extracellular pH was measured as 7.32±0.04 when using OVS, as compared to 7.39±0.03 when measured without OVS (N=7 subjects). Conclusion: The extracellular Pi signal acquired from the human head using nonlocalized 31P MRS contains a significant component likely contributed by peripheral blood and CSF in meninges that must be removed in order to use this signal as an endogenous probe for measuring extracellular pH and other properties in the brain.

Original languageEnglish (US)
JournalMagnetic Resonance in Medicine
DOIs
StateAccepted/In press - Jan 1 2018

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Phosphates
Head
Brain
Meninges

Keywords

  • P MRS
  • Blood
  • Brain
  • CSF
  • Inorganic phosphate
  • PH

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

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title = "Unveiling a hidden 31P signal coresonating with extracellular inorganic phosphate by outer-volume-suppression and localized 31P MRS in the human brain at 7T",
abstract = "Purpose: The study was undertaken to demonstrate that there is more than 1 component in the extracellular Pi 31P signal ( Piex) acquired from human head using nonlocalized 31P MRS. Methods: Outer-volume-suppression (OVS) saturation and 1D/2D 31P CSI were utilized to reveal the presence of an additional component in the Piex signal. Results: 67{\%} of the head extracellular Pi signal was attenuated upon OVS saturation of the peripheral meningeal tissues, likely reflecting elimination of the Pi signal in the meningeal fluids (the blood and CSF). Localized 1D/2D CSI data provided further support for this assignment. Upon correction for the meningeal contribution, the extracellular Pi concentration was 0.51±0.07mM, whereas the intracellular Pi was 0.85±0.10mM. The extracellular pH was measured as 7.32±0.04 when using OVS, as compared to 7.39±0.03 when measured without OVS (N=7 subjects). Conclusion: The extracellular Pi signal acquired from the human head using nonlocalized 31P MRS contains a significant component likely contributed by peripheral blood and CSF in meninges that must be removed in order to use this signal as an endogenous probe for measuring extracellular pH and other properties in the brain.",
keywords = "P MRS, Blood, Brain, CSF, Inorganic phosphate, PH",
author = "Jimin Ren and Ty Shang and Sherry, {A. Dean} and Malloy, {Craig R.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1002/mrm.27121",
language = "English (US)",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",

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T1 - Unveiling a hidden 31P signal coresonating with extracellular inorganic phosphate by outer-volume-suppression and localized 31P MRS in the human brain at 7T

AU - Ren, Jimin

AU - Shang, Ty

AU - Sherry, A. Dean

AU - Malloy, Craig R.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Purpose: The study was undertaken to demonstrate that there is more than 1 component in the extracellular Pi 31P signal ( Piex) acquired from human head using nonlocalized 31P MRS. Methods: Outer-volume-suppression (OVS) saturation and 1D/2D 31P CSI were utilized to reveal the presence of an additional component in the Piex signal. Results: 67% of the head extracellular Pi signal was attenuated upon OVS saturation of the peripheral meningeal tissues, likely reflecting elimination of the Pi signal in the meningeal fluids (the blood and CSF). Localized 1D/2D CSI data provided further support for this assignment. Upon correction for the meningeal contribution, the extracellular Pi concentration was 0.51±0.07mM, whereas the intracellular Pi was 0.85±0.10mM. The extracellular pH was measured as 7.32±0.04 when using OVS, as compared to 7.39±0.03 when measured without OVS (N=7 subjects). Conclusion: The extracellular Pi signal acquired from the human head using nonlocalized 31P MRS contains a significant component likely contributed by peripheral blood and CSF in meninges that must be removed in order to use this signal as an endogenous probe for measuring extracellular pH and other properties in the brain.

AB - Purpose: The study was undertaken to demonstrate that there is more than 1 component in the extracellular Pi 31P signal ( Piex) acquired from human head using nonlocalized 31P MRS. Methods: Outer-volume-suppression (OVS) saturation and 1D/2D 31P CSI were utilized to reveal the presence of an additional component in the Piex signal. Results: 67% of the head extracellular Pi signal was attenuated upon OVS saturation of the peripheral meningeal tissues, likely reflecting elimination of the Pi signal in the meningeal fluids (the blood and CSF). Localized 1D/2D CSI data provided further support for this assignment. Upon correction for the meningeal contribution, the extracellular Pi concentration was 0.51±0.07mM, whereas the intracellular Pi was 0.85±0.10mM. The extracellular pH was measured as 7.32±0.04 when using OVS, as compared to 7.39±0.03 when measured without OVS (N=7 subjects). Conclusion: The extracellular Pi signal acquired from the human head using nonlocalized 31P MRS contains a significant component likely contributed by peripheral blood and CSF in meninges that must be removed in order to use this signal as an endogenous probe for measuring extracellular pH and other properties in the brain.

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KW - Inorganic phosphate

KW - PH

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