Abstract
Purpose: We identified differentially expressed genes in the rabbit bladder after partial outlet obstruction. Materials and Methods: Differential display polymerase chain reaction (PCR) was performed on smooth muscle tissue from normal, 2 and 6-week obstructed rabbit bladders. Semiquantitative reverse transcriptase PCR, Western and RNA blot analysis were done to confirm messenger RNA and protein up-regulation. Results: A signal transducing protein human tumor necrosis factor receptor associated factor 6 (TRAF6)-like protein was identified on differential display PCR. TRAF6-like protein was up-regulated in rabbit bladders after 2 weeks of partial outlet obstruction. Reverse transcriptase PCR demonstrated TRAF6-like protein in bladder muscle tissue and semiquantitative analysis confirmed up-regulation in 2-week obstructed tissue. These findings were confirmed by RNA and Western blot analysis. Conclusions: TRAF6-like protein is up-regulated during the early phase of bladder outlet obstruction in rabbits. To our knowledge involvement of this gene in bladder outlet obstruction has not been described previously. TRAF6 may have a role in the regulation of molecular changes during the early bladder response to outlet obstruction, such as the up-regulation of growth factors and proto-oncogenes. Further understanding of this signaling pathway and its role in bladder outlet obstruction may open new avenues for treating detrusor dysfunction.
Original language | English (US) |
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Pages (from-to) | 1289-1293 |
Number of pages | 5 |
Journal | Journal of Urology |
Volume | 165 |
Issue number | 4 |
DOIs | |
State | Published - 2001 |
Keywords
- Bladder neck obstruction
- Muscle smooth
- NF-kappa B
- Rabbits
- Receptors
- Tumor necrosis factor
ASJC Scopus subject areas
- Urology