Up-regulation of a gene homologous to the human tumor necrosis factor receptor associated factor 6 gene in the obstructed rabbit bladder determined by differential display polymerase chain reaction

F. C. Burkhard, G. E. Lemack, M. D. Alcorn, P. E. Zimmern, V. K. Lin, J. D. McConnell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: We identified differentially expressed genes in the rabbit bladder after partial outlet obstruction. Materials and Methods: Differential display polymerase chain reaction (PCR) was performed on smooth muscle tissue from normal, 2 and 6-week obstructed rabbit bladders. Semiquantitative reverse transcriptase PCR, Western and RNA blot analysis were done to confirm messenger RNA and protein up-regulation. Results: A signal transducing protein human tumor necrosis factor receptor associated factor 6 (TRAF6)-like protein was identified on differential display PCR. TRAF6-like protein was up-regulated in rabbit bladders after 2 weeks of partial outlet obstruction. Reverse transcriptase PCR demonstrated TRAF6-like protein in bladder muscle tissue and semiquantitative analysis confirmed up-regulation in 2-week obstructed tissue. These findings were confirmed by RNA and Western blot analysis. Conclusions: TRAF6-like protein is up-regulated during the early phase of bladder outlet obstruction in rabbits. To our knowledge involvement of this gene in bladder outlet obstruction has not been described previously. TRAF6 may have a role in the regulation of molecular changes during the early bladder response to outlet obstruction, such as the up-regulation of growth factors and proto-oncogenes. Further understanding of this signaling pathway and its role in bladder outlet obstruction may open new avenues for treating detrusor dysfunction.

Original languageEnglish (US)
Pages (from-to)1289-1293
Number of pages5
JournalJournal of Urology
Volume165
Issue number4
StatePublished - 2001

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Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
Urinary Bladder
Up-Regulation
Rabbits
Urinary Bladder Neck Obstruction
Polymerase Chain Reaction
Genes
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Western Blotting
RNA
Muscles
Proto-Oncogenes
Smooth Muscle
human TNF protein
Intercellular Signaling Peptides and Proteins
Messenger RNA

Keywords

  • Bladder neck obstruction
  • Muscle smooth
  • NF-kappa B
  • Rabbits
  • Receptors
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Urology

Cite this

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title = "Up-regulation of a gene homologous to the human tumor necrosis factor receptor associated factor 6 gene in the obstructed rabbit bladder determined by differential display polymerase chain reaction",
abstract = "Purpose: We identified differentially expressed genes in the rabbit bladder after partial outlet obstruction. Materials and Methods: Differential display polymerase chain reaction (PCR) was performed on smooth muscle tissue from normal, 2 and 6-week obstructed rabbit bladders. Semiquantitative reverse transcriptase PCR, Western and RNA blot analysis were done to confirm messenger RNA and protein up-regulation. Results: A signal transducing protein human tumor necrosis factor receptor associated factor 6 (TRAF6)-like protein was identified on differential display PCR. TRAF6-like protein was up-regulated in rabbit bladders after 2 weeks of partial outlet obstruction. Reverse transcriptase PCR demonstrated TRAF6-like protein in bladder muscle tissue and semiquantitative analysis confirmed up-regulation in 2-week obstructed tissue. These findings were confirmed by RNA and Western blot analysis. Conclusions: TRAF6-like protein is up-regulated during the early phase of bladder outlet obstruction in rabbits. To our knowledge involvement of this gene in bladder outlet obstruction has not been described previously. TRAF6 may have a role in the regulation of molecular changes during the early bladder response to outlet obstruction, such as the up-regulation of growth factors and proto-oncogenes. Further understanding of this signaling pathway and its role in bladder outlet obstruction may open new avenues for treating detrusor dysfunction.",
keywords = "Bladder neck obstruction, Muscle smooth, NF-kappa B, Rabbits, Receptors, Tumor necrosis factor",
author = "Burkhard, {F. C.} and Lemack, {G. E.} and Alcorn, {M. D.} and Zimmern, {P. E.} and Lin, {V. K.} and McConnell, {J. D.}",
year = "2001",
language = "English (US)",
volume = "165",
pages = "1289--1293",
journal = "Journal of Urology",
issn = "0022-5347",
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number = "4",

}

TY - JOUR

T1 - Up-regulation of a gene homologous to the human tumor necrosis factor receptor associated factor 6 gene in the obstructed rabbit bladder determined by differential display polymerase chain reaction

AU - Burkhard, F. C.

AU - Lemack, G. E.

AU - Alcorn, M. D.

AU - Zimmern, P. E.

AU - Lin, V. K.

AU - McConnell, J. D.

PY - 2001

Y1 - 2001

N2 - Purpose: We identified differentially expressed genes in the rabbit bladder after partial outlet obstruction. Materials and Methods: Differential display polymerase chain reaction (PCR) was performed on smooth muscle tissue from normal, 2 and 6-week obstructed rabbit bladders. Semiquantitative reverse transcriptase PCR, Western and RNA blot analysis were done to confirm messenger RNA and protein up-regulation. Results: A signal transducing protein human tumor necrosis factor receptor associated factor 6 (TRAF6)-like protein was identified on differential display PCR. TRAF6-like protein was up-regulated in rabbit bladders after 2 weeks of partial outlet obstruction. Reverse transcriptase PCR demonstrated TRAF6-like protein in bladder muscle tissue and semiquantitative analysis confirmed up-regulation in 2-week obstructed tissue. These findings were confirmed by RNA and Western blot analysis. Conclusions: TRAF6-like protein is up-regulated during the early phase of bladder outlet obstruction in rabbits. To our knowledge involvement of this gene in bladder outlet obstruction has not been described previously. TRAF6 may have a role in the regulation of molecular changes during the early bladder response to outlet obstruction, such as the up-regulation of growth factors and proto-oncogenes. Further understanding of this signaling pathway and its role in bladder outlet obstruction may open new avenues for treating detrusor dysfunction.

AB - Purpose: We identified differentially expressed genes in the rabbit bladder after partial outlet obstruction. Materials and Methods: Differential display polymerase chain reaction (PCR) was performed on smooth muscle tissue from normal, 2 and 6-week obstructed rabbit bladders. Semiquantitative reverse transcriptase PCR, Western and RNA blot analysis were done to confirm messenger RNA and protein up-regulation. Results: A signal transducing protein human tumor necrosis factor receptor associated factor 6 (TRAF6)-like protein was identified on differential display PCR. TRAF6-like protein was up-regulated in rabbit bladders after 2 weeks of partial outlet obstruction. Reverse transcriptase PCR demonstrated TRAF6-like protein in bladder muscle tissue and semiquantitative analysis confirmed up-regulation in 2-week obstructed tissue. These findings were confirmed by RNA and Western blot analysis. Conclusions: TRAF6-like protein is up-regulated during the early phase of bladder outlet obstruction in rabbits. To our knowledge involvement of this gene in bladder outlet obstruction has not been described previously. TRAF6 may have a role in the regulation of molecular changes during the early bladder response to outlet obstruction, such as the up-regulation of growth factors and proto-oncogenes. Further understanding of this signaling pathway and its role in bladder outlet obstruction may open new avenues for treating detrusor dysfunction.

KW - Bladder neck obstruction

KW - Muscle smooth

KW - NF-kappa B

KW - Rabbits

KW - Receptors

KW - Tumor necrosis factor

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