Upregulation of b-FGF Receptor Expression after Carotid Bypass

Thomas E. Brothers, Jacob G. Robison, Bruce M. Elliott, Janet M. Boggs, Arthur E. Frankel, Mark C. Willingham

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Basic fibroblast growth factor (b-FGF) appears to be an important positive modulator of the neointimal hyperplasia that occurs after prosthetic vascular graft implantation through its effects on vascular myointimal/smooth muscle cell migration and proliferation. The distribution and extent of b-FGF receptor (b-FGFR1) expression was compared using immunohistochemical techniques in normal porcine carotid arteries and at various times up to 6 weeks following implantation of small caliber prosthetic vascular grafts. At the time of graft harvest, specimens were infused with OCT medium at 100 mm Hg and rapidly frozen in liquid nitrogen. Transverse sections of the perianastomotic arterial tissues were labeled with primary mouse monoclonal antibody directed toward the extracellular domain of the receptor, followed by goat-anti mouse IgG and rabbit anti-goat IgG conjugated to horseradish peroxiduse. The b-FGFR1-positive cells were identified by peroxidase activity within the Golgi complex of smooth muscle cells. Normal porcine carotid arteries showed no evidence of staining for b-FGFR1. However, at 6 weeks cells in the perianastomotic area clearly showed significant b-FGFR1 localization. Anti-muscle actin labeling confirmed these to be smooth muscle cells. The observed upregulation of b-FGFR1 expression supports the concept of positive feedback by cytokines as a contributing factor to the hyperplastic response of smooth muscle cells after prosthetic vascular graft implantation. This finding further supports a potential strategy to specifically target activated smooth muscle cells through use of mitotoxin therapy.

Original languageEnglish (US)
Pages (from-to)28-32
Number of pages5
JournalJournal of Surgical Research
Volume58
Issue number1
DOIs
StatePublished - Jan 1995

Fingerprint

Fibroblast Growth Factor Receptors
Fibroblast Growth Factor 2
Smooth Muscle Myocytes
Up-Regulation
Transplants
Blood Vessels
Carotid Arteries
Goats
Swine
Armoracia
Golgi Apparatus
Vascular Smooth Muscle
Peroxidase
Cell Movement
Hyperplasia
Actins
Nitrogen
Monoclonal Antibodies
Cell Proliferation
Staining and Labeling

ASJC Scopus subject areas

  • Surgery

Cite this

Brothers, T. E., Robison, J. G., Elliott, B. M., Boggs, J. M., Frankel, A. E., & Willingham, M. C. (1995). Upregulation of b-FGF Receptor Expression after Carotid Bypass. Journal of Surgical Research, 58(1), 28-32. https://doi.org/10.1006/jsre.1995.1005

Upregulation of b-FGF Receptor Expression after Carotid Bypass. / Brothers, Thomas E.; Robison, Jacob G.; Elliott, Bruce M.; Boggs, Janet M.; Frankel, Arthur E.; Willingham, Mark C.

In: Journal of Surgical Research, Vol. 58, No. 1, 01.1995, p. 28-32.

Research output: Contribution to journalArticle

Brothers, TE, Robison, JG, Elliott, BM, Boggs, JM, Frankel, AE & Willingham, MC 1995, 'Upregulation of b-FGF Receptor Expression after Carotid Bypass', Journal of Surgical Research, vol. 58, no. 1, pp. 28-32. https://doi.org/10.1006/jsre.1995.1005
Brothers, Thomas E. ; Robison, Jacob G. ; Elliott, Bruce M. ; Boggs, Janet M. ; Frankel, Arthur E. ; Willingham, Mark C. / Upregulation of b-FGF Receptor Expression after Carotid Bypass. In: Journal of Surgical Research. 1995 ; Vol. 58, No. 1. pp. 28-32.
@article{287cff90e93c491581afcc891e933967,
title = "Upregulation of b-FGF Receptor Expression after Carotid Bypass",
abstract = "Basic fibroblast growth factor (b-FGF) appears to be an important positive modulator of the neointimal hyperplasia that occurs after prosthetic vascular graft implantation through its effects on vascular myointimal/smooth muscle cell migration and proliferation. The distribution and extent of b-FGF receptor (b-FGFR1) expression was compared using immunohistochemical techniques in normal porcine carotid arteries and at various times up to 6 weeks following implantation of small caliber prosthetic vascular grafts. At the time of graft harvest, specimens were infused with OCT medium at 100 mm Hg and rapidly frozen in liquid nitrogen. Transverse sections of the perianastomotic arterial tissues were labeled with primary mouse monoclonal antibody directed toward the extracellular domain of the receptor, followed by goat-anti mouse IgG and rabbit anti-goat IgG conjugated to horseradish peroxiduse. The b-FGFR1-positive cells were identified by peroxidase activity within the Golgi complex of smooth muscle cells. Normal porcine carotid arteries showed no evidence of staining for b-FGFR1. However, at 6 weeks cells in the perianastomotic area clearly showed significant b-FGFR1 localization. Anti-muscle actin labeling confirmed these to be smooth muscle cells. The observed upregulation of b-FGFR1 expression supports the concept of positive feedback by cytokines as a contributing factor to the hyperplastic response of smooth muscle cells after prosthetic vascular graft implantation. This finding further supports a potential strategy to specifically target activated smooth muscle cells through use of mitotoxin therapy.",
author = "Brothers, {Thomas E.} and Robison, {Jacob G.} and Elliott, {Bruce M.} and Boggs, {Janet M.} and Frankel, {Arthur E.} and Willingham, {Mark C.}",
year = "1995",
month = "1",
doi = "10.1006/jsre.1995.1005",
language = "English (US)",
volume = "58",
pages = "28--32",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Upregulation of b-FGF Receptor Expression after Carotid Bypass

AU - Brothers, Thomas E.

AU - Robison, Jacob G.

AU - Elliott, Bruce M.

AU - Boggs, Janet M.

AU - Frankel, Arthur E.

AU - Willingham, Mark C.

PY - 1995/1

Y1 - 1995/1

N2 - Basic fibroblast growth factor (b-FGF) appears to be an important positive modulator of the neointimal hyperplasia that occurs after prosthetic vascular graft implantation through its effects on vascular myointimal/smooth muscle cell migration and proliferation. The distribution and extent of b-FGF receptor (b-FGFR1) expression was compared using immunohistochemical techniques in normal porcine carotid arteries and at various times up to 6 weeks following implantation of small caliber prosthetic vascular grafts. At the time of graft harvest, specimens were infused with OCT medium at 100 mm Hg and rapidly frozen in liquid nitrogen. Transverse sections of the perianastomotic arterial tissues were labeled with primary mouse monoclonal antibody directed toward the extracellular domain of the receptor, followed by goat-anti mouse IgG and rabbit anti-goat IgG conjugated to horseradish peroxiduse. The b-FGFR1-positive cells were identified by peroxidase activity within the Golgi complex of smooth muscle cells. Normal porcine carotid arteries showed no evidence of staining for b-FGFR1. However, at 6 weeks cells in the perianastomotic area clearly showed significant b-FGFR1 localization. Anti-muscle actin labeling confirmed these to be smooth muscle cells. The observed upregulation of b-FGFR1 expression supports the concept of positive feedback by cytokines as a contributing factor to the hyperplastic response of smooth muscle cells after prosthetic vascular graft implantation. This finding further supports a potential strategy to specifically target activated smooth muscle cells through use of mitotoxin therapy.

AB - Basic fibroblast growth factor (b-FGF) appears to be an important positive modulator of the neointimal hyperplasia that occurs after prosthetic vascular graft implantation through its effects on vascular myointimal/smooth muscle cell migration and proliferation. The distribution and extent of b-FGF receptor (b-FGFR1) expression was compared using immunohistochemical techniques in normal porcine carotid arteries and at various times up to 6 weeks following implantation of small caliber prosthetic vascular grafts. At the time of graft harvest, specimens were infused with OCT medium at 100 mm Hg and rapidly frozen in liquid nitrogen. Transverse sections of the perianastomotic arterial tissues were labeled with primary mouse monoclonal antibody directed toward the extracellular domain of the receptor, followed by goat-anti mouse IgG and rabbit anti-goat IgG conjugated to horseradish peroxiduse. The b-FGFR1-positive cells were identified by peroxidase activity within the Golgi complex of smooth muscle cells. Normal porcine carotid arteries showed no evidence of staining for b-FGFR1. However, at 6 weeks cells in the perianastomotic area clearly showed significant b-FGFR1 localization. Anti-muscle actin labeling confirmed these to be smooth muscle cells. The observed upregulation of b-FGFR1 expression supports the concept of positive feedback by cytokines as a contributing factor to the hyperplastic response of smooth muscle cells after prosthetic vascular graft implantation. This finding further supports a potential strategy to specifically target activated smooth muscle cells through use of mitotoxin therapy.

UR - http://www.scopus.com/inward/record.url?scp=0028887927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028887927&partnerID=8YFLogxK

U2 - 10.1006/jsre.1995.1005

DO - 10.1006/jsre.1995.1005

M3 - Article

C2 - 7830402

AN - SCOPUS:0028887927

VL - 58

SP - 28

EP - 32

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 1

ER -