Uptake and processing of dopamine by cells of the anterior pituitary gland

G. A. Gudelsky, D. D. Nansel, J. C. Porter

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The capacity of cells of the anterior pituitary gland to internalize dopamine and incorporate it into subcellar particles was investigated in vivo and under in vitro conditions. Most (75-85%) of the dopamine in the anterior pituitary gland was found to be associated with dense subcellular particles that could not be distinguished from particles, i.e. secretory granules, containing PRL. The amount of dopamine associated with the dense particles was greater in anterior pituitary glands from rats which had been treated with the dopamine precursor, L-dihydroxyphenylalanine (L-dopa), than in anterior pituitary glands from untreated animals. The effect of L-dopa was attenuated when the rats were pretreated with the dopamine agonist, bromocriptine. It was found that incubation of anterior pituitary glands with dopamine resulted in a dose-related increase in the amount of dopamine associated with the dense subcellular particles and that the incorporation of dopamine into these particles occurred within 15 min. Preincubation of anterior pituitary glands with the dopamine antagonist, cis-flupenthixol, or the dopamine agonist, perebedil, prevented the incorporation of dopamine into dense particles, suggestive of the view that this incorporation involves a dopamine receptor. It is concluded that cells of the anterior pituitary gland have the capacity to internalize dopamine and incorporate it into dense subcellular particles. In addition, it is suggested that these dopamine-containing particles may be PRL secretory granules.

Original languageEnglish (US)
Pages (from-to)30-34
Number of pages5
JournalEndocrinology
Volume107
Issue number1
StatePublished - 1980

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Anterior Pituitary Gland
Dopamine
Dihydroxyphenylalanine
Dopamine Agonists
Secretory Vesicles
Flupenthixol
Bromocriptine
Dopamine Antagonists
Dopamine Receptors

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Gudelsky, G. A., Nansel, D. D., & Porter, J. C. (1980). Uptake and processing of dopamine by cells of the anterior pituitary gland. Endocrinology, 107(1), 30-34.

Uptake and processing of dopamine by cells of the anterior pituitary gland. / Gudelsky, G. A.; Nansel, D. D.; Porter, J. C.

In: Endocrinology, Vol. 107, No. 1, 1980, p. 30-34.

Research output: Contribution to journalArticle

Gudelsky, GA, Nansel, DD & Porter, JC 1980, 'Uptake and processing of dopamine by cells of the anterior pituitary gland', Endocrinology, vol. 107, no. 1, pp. 30-34.
Gudelsky, G. A. ; Nansel, D. D. ; Porter, J. C. / Uptake and processing of dopamine by cells of the anterior pituitary gland. In: Endocrinology. 1980 ; Vol. 107, No. 1. pp. 30-34.
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