Urea extraction of Z bands, intercalated disks, and desmosomes

John E. Rash, Jerry W. Shay, John J. Biesele

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Specific extraction of Z bands, intercalated disks and desmosomes was accomplished with molar concentrations of urea. Thick and thin filaments and M line material were undisturbed, demonstrating that the Z band protein is not similar to actin, myosin, or the M-line protein. The literature indicates that the tropomyosins are the only other structural proteins present in sufficient amounts to account for the extracted Z bands. However, the evidence for a lipid-protein association in the Z bands is considered and is further supported by the close association of the dense material of the Z bands, intercalated disks, and desmosomes with the lipoprotein cell membranes.

Original languageEnglish (US)
Pages (from-to)181-189
Number of pages9
JournalJournal of Ultrasructure Research
Volume24
Issue number3-4
StatePublished - Aug 1968

Fingerprint

Desmosomes
Urea
Tropomyosin
Proteins
Myosins
Lipoproteins
Actins
Cell Membrane
Lipids

ASJC Scopus subject areas

  • Anatomy
  • Molecular Biology

Cite this

Urea extraction of Z bands, intercalated disks, and desmosomes. / Rash, John E.; Shay, Jerry W.; Biesele, John J.

In: Journal of Ultrasructure Research, Vol. 24, No. 3-4, 08.1968, p. 181-189.

Research output: Contribution to journalArticle

Rash, John E. ; Shay, Jerry W. ; Biesele, John J. / Urea extraction of Z bands, intercalated disks, and desmosomes. In: Journal of Ultrasructure Research. 1968 ; Vol. 24, No. 3-4. pp. 181-189.
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