Ureteral function is modulated by a local renin-angiotensin system

William F. Santis, Craig A Peters, Subbarao V. Yalla, Maryrose P. Sullivan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: Although many aspects of ureteral physiology are well characterized, the exact mechanism of ureteral smooth muscle modulation has not been fully established. In other smooth muscle contractility is modulated by angiotensin II (AngII). We determined the presence of a local ureteral renin-angiotensin system and characterized the functional role of AngII in ureteral smooth muscle. Materials and Methods: Reverse transcriptase-polymerase chain reaction was performed to determine the expression of angiotensinogen, renin, angiotensin-converting enzyme and angiotensin receptor subtype 1 mRNA. The presence of AngII in ureteral tissue was determined by immunohistochemistry. Human and pig ureteral smooth muscle strips were suspended in tissue baths to determine the effect of the AnglI Angll receptor antagonist losartan on the frequency and amplitude of spontaneous ureteral contractions. Electrical field stimulation was performed before and after exposure to losartan. Results: Angiotensinogen, renin, angiotensin-converting enzyme and angiotensin receptor subtype 1 mRNA expression was detected in human ureter. Immunoreactivity for AngII was demonstrated in smooth muscle bundles and blood vessels of the ureter. Losartan decreased the amplitude and frequency of spontaneous ureteral contractions as well as the contractile response to electrical field stimulation in a dose dependent manner. Conclusions: Gene expression of AngII precursors and receptor, and localization of AngII in the ureter suggest the presence of a local renin-angiotensin system in the ureter. The effect of AngII receptor antagonist on contractile responses suggests that AngII modulates ureteral smooth muscle contractile function. Therefore, a local renin-angiotensin system may have an important role in ureteral function under physiological and pathological conditions.

Original languageEnglish (US)
Pages (from-to)259-263
Number of pages5
JournalJournal of Urology
Volume170
Issue number1
DOIs
StatePublished - Jul 1 2003

Fingerprint

Renin-Angiotensin System
Angiotensin II
Smooth Muscle
Ureter
Angiotensin Receptors
Losartan
Angiotensinogen
Peptidyl-Dipeptidase A
Renin
Electric Stimulation
Messenger RNA
Angiotensin Receptor Antagonists
Reverse Transcriptase Polymerase Chain Reaction
Baths
Blood Vessels
Swine
Immunohistochemistry
Gene Expression

Keywords

  • Angiotensin II
  • Angiotensinogen
  • Muscle, smooth
  • Ureter

ASJC Scopus subject areas

  • Urology

Cite this

Ureteral function is modulated by a local renin-angiotensin system. / Santis, William F.; Peters, Craig A; Yalla, Subbarao V.; Sullivan, Maryrose P.

In: Journal of Urology, Vol. 170, No. 1, 01.07.2003, p. 259-263.

Research output: Contribution to journalArticle

Santis, William F. ; Peters, Craig A ; Yalla, Subbarao V. ; Sullivan, Maryrose P. / Ureteral function is modulated by a local renin-angiotensin system. In: Journal of Urology. 2003 ; Vol. 170, No. 1. pp. 259-263.
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