Ureteral replacement using porcine small intestine submucosa in a porcine model

Thomas G. Smith, Matthew Gettman, Guy Lindberg, Cheryl Napper, Margaret S Pearle, Jeffrey A Cadeddu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Objectives. To investigate an alternative technique using an onlay patch of porcine small intestine submucosa (SIS) allograft to bridge a ureteral defect. For ureteral strictures that fail endourologic management, few options are available for minimally invasive repair or reconstruction. Although laparoscopic interposition of a tubularized allograft of porcine SIS has great promise, animal studies have yielded mixed results. Methods. In 9 anesthetized female pigs, cystoscopy and retrograde pyelography were performed, and a ureteral stent was placed. Transperitoneal laparoscopic access was obtained, and a segment of ureter 2 cm long and encompassing one half the ureteral circumference was excised. An oval-shaped patch of SIS was sutured to the native ureter to cover the defect. In one control survival animal, the ureter was excised and a stent placed, but no SIS onlay was performed. Two pigs were killed immediately. In the survival group (6 pigs), the stents were removed at 1 week (n = 2), 2 weeks (n = 2), or 4 weeks (n = 2) and the corresponding animals were killed at 3 weeks (n = 2), 6 weeks (n = 2), and 9 weeks (n = 2). Intravenous urography was performed to evaluate renal function, and retrograde pyelography was performed after harvest to identify ureteral stricture or obstruction. The ureteral grafts were measured and examined histologically. Results. All 6 kidneys from the survival group were grossly normal, appeared promptly on intravenous urography, and were patent on retrograde pyelography. The control animal demonstrated complete ureteral obstruction. By 9 weeks, the SIS graft was replaced with ureteral tissue, including the muscle layers. The epithelium was primarily transitional epithelium, with focal intestinal metaplasia. The submucosa and ureteral musculature appeared histologically normal. Conclusions. In the porcine model, a patch graft technique using SIS appears to induce ureteral regrowth. Renal function remained intact, and no evidence of stricture was demonstrated on radiographic imaging. Before clinical application of this technique, evaluation in a stricture model is required.

Original languageEnglish (US)
Pages (from-to)931-934
Number of pages4
JournalUrology
Volume60
Issue number5
DOIs
StatePublished - Nov 1 2002

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Urography
Small Intestine
Swine
Pathologic Constriction
Ureter
Stents
Inlays
Transplants
Kidney
Allografts
Epithelium
Ureteral Obstruction
Cystoscopy
Metaplasia
Muscles

ASJC Scopus subject areas

  • Urology

Cite this

Ureteral replacement using porcine small intestine submucosa in a porcine model. / Smith, Thomas G.; Gettman, Matthew; Lindberg, Guy; Napper, Cheryl; Pearle, Margaret S; Cadeddu, Jeffrey A.

In: Urology, Vol. 60, No. 5, 01.11.2002, p. 931-934.

Research output: Contribution to journalArticle

Smith, Thomas G. ; Gettman, Matthew ; Lindberg, Guy ; Napper, Cheryl ; Pearle, Margaret S ; Cadeddu, Jeffrey A. / Ureteral replacement using porcine small intestine submucosa in a porcine model. In: Urology. 2002 ; Vol. 60, No. 5. pp. 931-934.
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abstract = "Objectives. To investigate an alternative technique using an onlay patch of porcine small intestine submucosa (SIS) allograft to bridge a ureteral defect. For ureteral strictures that fail endourologic management, few options are available for minimally invasive repair or reconstruction. Although laparoscopic interposition of a tubularized allograft of porcine SIS has great promise, animal studies have yielded mixed results. Methods. In 9 anesthetized female pigs, cystoscopy and retrograde pyelography were performed, and a ureteral stent was placed. Transperitoneal laparoscopic access was obtained, and a segment of ureter 2 cm long and encompassing one half the ureteral circumference was excised. An oval-shaped patch of SIS was sutured to the native ureter to cover the defect. In one control survival animal, the ureter was excised and a stent placed, but no SIS onlay was performed. Two pigs were killed immediately. In the survival group (6 pigs), the stents were removed at 1 week (n = 2), 2 weeks (n = 2), or 4 weeks (n = 2) and the corresponding animals were killed at 3 weeks (n = 2), 6 weeks (n = 2), and 9 weeks (n = 2). Intravenous urography was performed to evaluate renal function, and retrograde pyelography was performed after harvest to identify ureteral stricture or obstruction. The ureteral grafts were measured and examined histologically. Results. All 6 kidneys from the survival group were grossly normal, appeared promptly on intravenous urography, and were patent on retrograde pyelography. The control animal demonstrated complete ureteral obstruction. By 9 weeks, the SIS graft was replaced with ureteral tissue, including the muscle layers. The epithelium was primarily transitional epithelium, with focal intestinal metaplasia. The submucosa and ureteral musculature appeared histologically normal. Conclusions. In the porcine model, a patch graft technique using SIS appears to induce ureteral regrowth. Renal function remained intact, and no evidence of stricture was demonstrated on radiographic imaging. Before clinical application of this technique, evaluation in a stricture model is required.",
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