Uroguanylin: Cloning of preprouroguanylin cDNA, mRNA expression in the intestine and heart and isolation of uroguanylin and prouroguanylin from plasma

Xiaohui Fan, F. Kent Hamra, Ronald H. Freeman, Sammy L. Eber, William J. Krause, Robert W. Lim, Victor M. Pace, Mark G. Currie, Leonard R. Forte

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Uroguanylin is a small peptide isolated from opossum urine that activates membrane guanylate cyclases. We report the isolation by molecular cloning of cDNAs encoding the 109 amino acid preprouroguanylin containing the active uroguanylin peptide at its C-terminus. Preprouroguanylin mRNAs of 1.2 kb were detected throughout the small and large intestine and in the atria and ventricles of heart, but not in kidney, stomach or liver. Transfection of COS-1 cells with the uroguanylin cDNA resulted in prouroguanylin secretion. Both uroguanylin and prouroguanylin were isolated from opossum plasma. Thus, uroguanylin is made by the intestine and heart and circulates as a bioactive form of uroguanylin and the inactive prouroguanylin.

Original languageEnglish (US)
Pages (from-to)457-462
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume219
Issue number2
DOIs
StatePublished - Feb 15 1996

Fingerprint

Cloning
Intestines
Organism Cloning
Complementary DNA
Plasmas
Messenger RNA
Opossums
Peptides
Guanylate Cyclase
COS Cells
Large Intestine
Molecular Cloning
Heart Atria
Liver
Small Intestine
Heart Ventricles
Transfection
prouroguanylin
uroguanylin
Stomach

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Uroguanylin : Cloning of preprouroguanylin cDNA, mRNA expression in the intestine and heart and isolation of uroguanylin and prouroguanylin from plasma. / Fan, Xiaohui; Hamra, F. Kent; Freeman, Ronald H.; Eber, Sammy L.; Krause, William J.; Lim, Robert W.; Pace, Victor M.; Currie, Mark G.; Forte, Leonard R.

In: Biochemical and Biophysical Research Communications, Vol. 219, No. 2, 15.02.1996, p. 457-462.

Research output: Contribution to journalArticle

Fan, Xiaohui ; Hamra, F. Kent ; Freeman, Ronald H. ; Eber, Sammy L. ; Krause, William J. ; Lim, Robert W. ; Pace, Victor M. ; Currie, Mark G. ; Forte, Leonard R. / Uroguanylin : Cloning of preprouroguanylin cDNA, mRNA expression in the intestine and heart and isolation of uroguanylin and prouroguanylin from plasma. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 219, No. 2. pp. 457-462.
@article{91666ce94a5b44dfa66bf507dd0a2728,
title = "Uroguanylin: Cloning of preprouroguanylin cDNA, mRNA expression in the intestine and heart and isolation of uroguanylin and prouroguanylin from plasma",
abstract = "Uroguanylin is a small peptide isolated from opossum urine that activates membrane guanylate cyclases. We report the isolation by molecular cloning of cDNAs encoding the 109 amino acid preprouroguanylin containing the active uroguanylin peptide at its C-terminus. Preprouroguanylin mRNAs of 1.2 kb were detected throughout the small and large intestine and in the atria and ventricles of heart, but not in kidney, stomach or liver. Transfection of COS-1 cells with the uroguanylin cDNA resulted in prouroguanylin secretion. Both uroguanylin and prouroguanylin were isolated from opossum plasma. Thus, uroguanylin is made by the intestine and heart and circulates as a bioactive form of uroguanylin and the inactive prouroguanylin.",
author = "Xiaohui Fan and Hamra, {F. Kent} and Freeman, {Ronald H.} and Eber, {Sammy L.} and Krause, {William J.} and Lim, {Robert W.} and Pace, {Victor M.} and Currie, {Mark G.} and Forte, {Leonard R.}",
year = "1996",
month = "2",
day = "15",
doi = "10.1006/bbrc.1996.0255",
language = "English (US)",
volume = "219",
pages = "457--462",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Uroguanylin

T2 - Cloning of preprouroguanylin cDNA, mRNA expression in the intestine and heart and isolation of uroguanylin and prouroguanylin from plasma

AU - Fan, Xiaohui

AU - Hamra, F. Kent

AU - Freeman, Ronald H.

AU - Eber, Sammy L.

AU - Krause, William J.

AU - Lim, Robert W.

AU - Pace, Victor M.

AU - Currie, Mark G.

AU - Forte, Leonard R.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Uroguanylin is a small peptide isolated from opossum urine that activates membrane guanylate cyclases. We report the isolation by molecular cloning of cDNAs encoding the 109 amino acid preprouroguanylin containing the active uroguanylin peptide at its C-terminus. Preprouroguanylin mRNAs of 1.2 kb were detected throughout the small and large intestine and in the atria and ventricles of heart, but not in kidney, stomach or liver. Transfection of COS-1 cells with the uroguanylin cDNA resulted in prouroguanylin secretion. Both uroguanylin and prouroguanylin were isolated from opossum plasma. Thus, uroguanylin is made by the intestine and heart and circulates as a bioactive form of uroguanylin and the inactive prouroguanylin.

AB - Uroguanylin is a small peptide isolated from opossum urine that activates membrane guanylate cyclases. We report the isolation by molecular cloning of cDNAs encoding the 109 amino acid preprouroguanylin containing the active uroguanylin peptide at its C-terminus. Preprouroguanylin mRNAs of 1.2 kb were detected throughout the small and large intestine and in the atria and ventricles of heart, but not in kidney, stomach or liver. Transfection of COS-1 cells with the uroguanylin cDNA resulted in prouroguanylin secretion. Both uroguanylin and prouroguanylin were isolated from opossum plasma. Thus, uroguanylin is made by the intestine and heart and circulates as a bioactive form of uroguanylin and the inactive prouroguanylin.

UR - http://www.scopus.com/inward/record.url?scp=0029985322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029985322&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1996.0255

DO - 10.1006/bbrc.1996.0255

M3 - Article

C2 - 8605009

AN - SCOPUS:0029985322

VL - 219

SP - 457

EP - 462

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -