USAG-1

A bone morphogenetic protein antagonist abundantly expressed in the kidney

Motoko Yanagita, Masako Oka, Tetsuro Watabe, Haruhisa Iguchi, Atsushi Niida, Satoru Takahashi, Tetsu Akiyama, Kohei Miyazono, Masashi Yanagisawa, Takeshi Sakurai

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Bone morphogenetic proteins (BMPs) play critical roles in cellular proliferation, differentiation, and programmed cell death in multiple tissues. An increasing body of recent evidence has suggested that classes of molecules collectively termed BMP antagonists play important roles for the local regulation of BMP actions by binding BMPs and neutralizing their activities. Uterine sensitization-associated gene-1 (USAG-1) was previously reported as a gene of unknown function, preferentially expressed in sensitized endometrium of the rat uterus. Here, we show that USAG-1 is abundantly expressed in the kidney and functions as a BMP antagonist. Recombinant USAG-1 binds directly to BMPs and antagonizes the BMP-mediated induction of alkaline phosphatase in C2C12 cells. USAG-1 also induces formation of secondary axis and/or hyperdorsalization when its mRNA is injected to Xenopus embryos. In the early stage of mouse embryogenesis, USAG-1 is expressed in the first and second branchial arches and in metanephros, while in later stages the expression is confined to renal tubules and ameloblasts of teeth. Postnatally, the expression is further restricted to distal tubules of kidney, in a pattern similar to the localization of BMP-7, which has been shown to be important in the development of kidney and preservation of adult renal functions under pathological stresses. Collectively, we suggest that USAG-1 is a BMP antagonist that interacts with BMP-7 in the developing and adult kidney.

Original languageEnglish (US)
Pages (from-to)490-500
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number2
DOIs
StatePublished - Apr 2 2004

Fingerprint

Bone Morphogenetic Protein 1
Bone Morphogenetic Proteins
Genes
Kidney
Bone Morphogenetic Protein 7
Distal Kidney Tubule
Ameloblasts
Branchial Region
Arches
Cell death
Endometrium
Xenopus
Protein Binding
Uterus
Embryonic Development
Alkaline Phosphatase
Rats
Tooth
Carrier Proteins
Cell Death

Keywords

  • Ectodin
  • Mouse
  • Sclerostin
  • Wise
  • Xenopus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yanagita, M., Oka, M., Watabe, T., Iguchi, H., Niida, A., Takahashi, S., ... Sakurai, T. (2004). USAG-1: A bone morphogenetic protein antagonist abundantly expressed in the kidney. Biochemical and Biophysical Research Communications, 316(2), 490-500. https://doi.org/10.1016/j.bbrc.2004.02.075

USAG-1 : A bone morphogenetic protein antagonist abundantly expressed in the kidney. / Yanagita, Motoko; Oka, Masako; Watabe, Tetsuro; Iguchi, Haruhisa; Niida, Atsushi; Takahashi, Satoru; Akiyama, Tetsu; Miyazono, Kohei; Yanagisawa, Masashi; Sakurai, Takeshi.

In: Biochemical and Biophysical Research Communications, Vol. 316, No. 2, 02.04.2004, p. 490-500.

Research output: Contribution to journalArticle

Yanagita, M, Oka, M, Watabe, T, Iguchi, H, Niida, A, Takahashi, S, Akiyama, T, Miyazono, K, Yanagisawa, M & Sakurai, T 2004, 'USAG-1: A bone morphogenetic protein antagonist abundantly expressed in the kidney', Biochemical and Biophysical Research Communications, vol. 316, no. 2, pp. 490-500. https://doi.org/10.1016/j.bbrc.2004.02.075
Yanagita, Motoko ; Oka, Masako ; Watabe, Tetsuro ; Iguchi, Haruhisa ; Niida, Atsushi ; Takahashi, Satoru ; Akiyama, Tetsu ; Miyazono, Kohei ; Yanagisawa, Masashi ; Sakurai, Takeshi. / USAG-1 : A bone morphogenetic protein antagonist abundantly expressed in the kidney. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 316, No. 2. pp. 490-500.
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