Use of a multi-compartment dynamic single enzyme phantom for studies of hyperpolarized magnetic resonance agents

Christopher M. Walker, Matthew Merritt, Jian Xiong Wang, James A. Bankson

Research output: Contribution to journalArticle

Abstract

Imaging of hyperpolarized substrates by magnetic resonance shows great clinical promise for assessment of critical biochemical processes in real time. Due to fundamental constraints imposed by the hyperpolarized state, exotic imaging and reconstruction techniques are commonly used. A practical system for characterization of dynamic, multi-spectral imaging methods is critically needed. Such a system must reproducibly recapitulate the relevant chemical dynamics of normal and pathological tissues. The most widely utilized substrate to date is hyperpolarized [1-13C]-pyruvate for assessment of cancer metabolism. We describe an enzyme-based phantom system that mediates the conversion of pyruvate to lactate. The reaction is initiated by injection of the hyperpolarized agent into multiple chambers within the phantom, each of which contains varying concentrations of reagents that control the reaction rate. Multiple compartments are necessary to ensure that imaging sequences faithfully capture the spatial and metabolic heterogeneity of tissue. This system will aid the development and validation of advanced imaging strategies by providing chemical dynamics that are not available from conventional phantoms, as well as control and reproducibility that is not possible in vivo.

Original languageEnglish (US)
Article numbere53607
JournalJournal of Visualized Experiments
Volume2016
Issue number110
DOIs
StatePublished - Apr 1 2016

Keywords

  • Carbon 13
  • Hyperpolarized
  • Issue 110
  • Magnetic resonance imaging
  • Medicine
  • Phantom
  • Pyruvate
  • Quality assurance
  • Sequence development

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

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