Use of a single 13C NMR resonance of glutamate for measuring oxygen consumption in tissue

F. Mark H Jeffrey, Alexander Reshetov, Charles J. Storey, Rui A. Carvalho, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Akinetic model of the citric acid cycle for calculating oxygen consumption from 13C nuclear magnetic resonance (NMR) multiplet data has been developed. Measured oxygen consumption (MV̇O2) was compared with MV̇O2 predicted by the model with 13C NMR data obtained from rat hearts perfused with glucose and either [2-13C]acetate or [3-13C]pyruvate. The accuracy of MV̇O2 measured from three subsets of NMR data was compared: glutamate C-4 and C-3 resonance areas; the doublet C4D34 (expressed as a fraction of C-4 area); and C-4 and C-3 areas plus several multiplets of C-2, C-3, and C-4. MV̇O2 determined by set 2 (C4D34 only) gave the same degree of accuracy as set 3 (complete data); both were superior to set 1 (C-4 and C- 3 areas). Analysis of the latter suffers from the correlation between citric acid cycle flux and exchange between α-ketoglutarate and glutamate, resulting in greater error in estimating MV̇O2. Analysis of C4D34 is less influenced by correlation between parameters, and this single measurement provides the best opportunity for a noninvasive measurement of oxygen consumption.

Original languageEnglish (US)
Pages (from-to)E1111-E1121
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume277
Issue number6 40-6
StatePublished - Dec 1 1999

Keywords

  • C isotopomer analysis
  • Citric acid cycle flux
  • Heart metabolism
  • Nuclear magnetic resonance

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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