Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow

M. Muirhead, P. J. Martin, B. Torok-Storb, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Affinity-purified rabbit antibody to human lambda and kappa chains (RαHλκ) was conjugated to the A-chain of the plant toxin, ricin. The resulting immunotoxin (RαHλκ-A) killed cells from the tumor cell line Daudi, which bears surface immunoglobulin, but was nontoxic to the CFU-E, BFU-E, and CFU-GM of human bone marrow. RαHλκ-A eliminated 99% of clonogenic Daudi cells that had been mixed with marrow cells in vitro, without demonstrable toxicity to hematopoietic cells. Thus, in vitro treatment of marrow with RαHλκ-A may increase the incidence of cure following autologous bone marrow transplantation for the treatment of human B-cell malignancies.

Original languageEnglish (US)
Pages (from-to)327-332
Number of pages6
JournalBlood
Volume62
Issue number2
StatePublished - 1983

Fingerprint

Ricin
Bone
B-Lymphocytes
Bone Marrow
Cells
Erythroid Precursor Cells
Antibodies
Immunotoxins
B-Cell Antigen Receptors
Granulocyte-Macrophage Progenitor Cells
Toxicity
Autologous Transplantation
Tumors
Tumor Cell Line
Bone Marrow Transplantation
Rabbits
Incidence
Therapeutics
Neoplasms
In Vitro Techniques

ASJC Scopus subject areas

  • Hematology

Cite this

Muirhead, M., Martin, P. J., Torok-Storb, B., Uhr, J. W., & Vitetta, E. S. (1983). Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow. Blood, 62(2), 327-332.

Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow. / Muirhead, M.; Martin, P. J.; Torok-Storb, B.; Uhr, J. W.; Vitetta, E. S.

In: Blood, Vol. 62, No. 2, 1983, p. 327-332.

Research output: Contribution to journalArticle

Muirhead, M, Martin, PJ, Torok-Storb, B, Uhr, JW & Vitetta, ES 1983, 'Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow', Blood, vol. 62, no. 2, pp. 327-332.
Muirhead, M. ; Martin, P. J. ; Torok-Storb, B. ; Uhr, J. W. ; Vitetta, E. S. / Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow. In: Blood. 1983 ; Vol. 62, No. 2. pp. 327-332.
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