Use of an antibody-ricin A-chain conjugate to delete neoplastic B cells from human bone marrow

M. Muirhead, P. J. Martin, B. Torok-Storb, J. W. Uhr, E. S. Vitetta

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Affinity-purified rabbit antibody to human lambda and kappa chains (RαHλκ) was conjugated to the A-chain of the plant toxin, ricin. The resulting immunotoxin (RαHλκ-A) killed cells from the tumor cell line Daudi, which bears surface immunoglobulin, but was nontoxic to the CFU-E, BFU-E, and CFU-GM of human bone marrow. RαHλκ-A eliminated 99% of clonogenic Daudi cells that had been mixed with marrow cells in vitro, without demonstrable toxicity to hematopoietic cells. Thus, in vitro treatment of marrow with RαHλκ-A may increase the incidence of cure following autologous bone marrow transplantation for the treatment of human B-cell malignancies.

Original languageEnglish (US)
Pages (from-to)327-332
Number of pages6
JournalUnknown Journal
Volume62
Issue number2
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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