Use of molecular probes to study regulation of aromatase cytochrome P-450

C. R. Mendelson, G. D. Means, M. S. Mahendroo, C. J. Corbin, M. P. Steinkampf, S. Graham-Lorence, E. R. Simpson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aromatase, an enzyme complex localized in the endoplasmic reticulum of estrogen-producing cells, is composed of NADPH-cytochrome P-450 reductase, and aromatase cytochrome P-450 (cytochrome P-450(AROM)). To define the molecular mechanisms involved in the multifactorial regulation of cytochrome P-450(AROM) in estrogen-producing cells, we have isolated a cDNA specific for human cytochrome P-450(AROM) and have used this cDNA to isolate the human cytochrome P-450(AROM) gene. The cDNA sequence encodes a polypeptide of 503 amino acids and contains - near the carboxy-terminus, a region of high homology with the putative heme-binding regions of other P-450 cytochromes. COS1 cells transfected with an expression plasmid containing the cytochrome P-450(AROM) cDNA had the capacity to aromatize testosterone, androstenedione and 16α-hydroxyandrostenedione, suggesting that a single polypeptide catalyzes all steps of the aromatization reaction using either of the three major C19-substrates. The human cytochrome P-450(AROM) gene is > 52 kb in size and consists of 10 exons and 9 introns. Hormonally induced changes in aromatase activity of human ovarian granulosa and adipose stromal cells are associated with comparable changes in cytochrome P-450(AROM) gene expression and synthesis, whereas the reductase component is only modestly affected. Studies are in progress to define the molecular mechanisms involved in the regulation of cytochrome P-450(AROM) gene expression in estrogen-produced cells.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalBiology of Reproduction
Volume42
Issue number1
StatePublished - 1990

Fingerprint

Molecular Probes
Aromatase
Cytochrome P-450 Enzyme System
Complementary DNA
Estrogens
Gene Expression
NADPH-Ferrihemoprotein Reductase
Peptides
Androstenedione
Stromal Cells
Heme
Human Activities
Endoplasmic Reticulum
Introns
Genes
Testosterone
Exons
Oxidoreductases
Plasmids

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Mendelson, C. R., Means, G. D., Mahendroo, M. S., Corbin, C. J., Steinkampf, M. P., Graham-Lorence, S., & Simpson, E. R. (1990). Use of molecular probes to study regulation of aromatase cytochrome P-450. Biology of Reproduction, 42(1), 1-10.

Use of molecular probes to study regulation of aromatase cytochrome P-450. / Mendelson, C. R.; Means, G. D.; Mahendroo, M. S.; Corbin, C. J.; Steinkampf, M. P.; Graham-Lorence, S.; Simpson, E. R.

In: Biology of Reproduction, Vol. 42, No. 1, 1990, p. 1-10.

Research output: Contribution to journalArticle

Mendelson, CR, Means, GD, Mahendroo, MS, Corbin, CJ, Steinkampf, MP, Graham-Lorence, S & Simpson, ER 1990, 'Use of molecular probes to study regulation of aromatase cytochrome P-450', Biology of Reproduction, vol. 42, no. 1, pp. 1-10.
Mendelson CR, Means GD, Mahendroo MS, Corbin CJ, Steinkampf MP, Graham-Lorence S et al. Use of molecular probes to study regulation of aromatase cytochrome P-450. Biology of Reproduction. 1990;42(1):1-10.
Mendelson, C. R. ; Means, G. D. ; Mahendroo, M. S. ; Corbin, C. J. ; Steinkampf, M. P. ; Graham-Lorence, S. ; Simpson, E. R. / Use of molecular probes to study regulation of aromatase cytochrome P-450. In: Biology of Reproduction. 1990 ; Vol. 42, No. 1. pp. 1-10.
@article{1f866d19136a47109652b0d6b56f7ce5,
title = "Use of molecular probes to study regulation of aromatase cytochrome P-450",
abstract = "Aromatase, an enzyme complex localized in the endoplasmic reticulum of estrogen-producing cells, is composed of NADPH-cytochrome P-450 reductase, and aromatase cytochrome P-450 (cytochrome P-450(AROM)). To define the molecular mechanisms involved in the multifactorial regulation of cytochrome P-450(AROM) in estrogen-producing cells, we have isolated a cDNA specific for human cytochrome P-450(AROM) and have used this cDNA to isolate the human cytochrome P-450(AROM) gene. The cDNA sequence encodes a polypeptide of 503 amino acids and contains - near the carboxy-terminus, a region of high homology with the putative heme-binding regions of other P-450 cytochromes. COS1 cells transfected with an expression plasmid containing the cytochrome P-450(AROM) cDNA had the capacity to aromatize testosterone, androstenedione and 16α-hydroxyandrostenedione, suggesting that a single polypeptide catalyzes all steps of the aromatization reaction using either of the three major C19-substrates. The human cytochrome P-450(AROM) gene is > 52 kb in size and consists of 10 exons and 9 introns. Hormonally induced changes in aromatase activity of human ovarian granulosa and adipose stromal cells are associated with comparable changes in cytochrome P-450(AROM) gene expression and synthesis, whereas the reductase component is only modestly affected. Studies are in progress to define the molecular mechanisms involved in the regulation of cytochrome P-450(AROM) gene expression in estrogen-produced cells.",
author = "Mendelson, {C. R.} and Means, {G. D.} and Mahendroo, {M. S.} and Corbin, {C. J.} and Steinkampf, {M. P.} and S. Graham-Lorence and Simpson, {E. R.}",
year = "1990",
language = "English (US)",
volume = "42",
pages = "1--10",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "1",

}

TY - JOUR

T1 - Use of molecular probes to study regulation of aromatase cytochrome P-450

AU - Mendelson, C. R.

AU - Means, G. D.

AU - Mahendroo, M. S.

AU - Corbin, C. J.

AU - Steinkampf, M. P.

AU - Graham-Lorence, S.

AU - Simpson, E. R.

PY - 1990

Y1 - 1990

N2 - Aromatase, an enzyme complex localized in the endoplasmic reticulum of estrogen-producing cells, is composed of NADPH-cytochrome P-450 reductase, and aromatase cytochrome P-450 (cytochrome P-450(AROM)). To define the molecular mechanisms involved in the multifactorial regulation of cytochrome P-450(AROM) in estrogen-producing cells, we have isolated a cDNA specific for human cytochrome P-450(AROM) and have used this cDNA to isolate the human cytochrome P-450(AROM) gene. The cDNA sequence encodes a polypeptide of 503 amino acids and contains - near the carboxy-terminus, a region of high homology with the putative heme-binding regions of other P-450 cytochromes. COS1 cells transfected with an expression plasmid containing the cytochrome P-450(AROM) cDNA had the capacity to aromatize testosterone, androstenedione and 16α-hydroxyandrostenedione, suggesting that a single polypeptide catalyzes all steps of the aromatization reaction using either of the three major C19-substrates. The human cytochrome P-450(AROM) gene is > 52 kb in size and consists of 10 exons and 9 introns. Hormonally induced changes in aromatase activity of human ovarian granulosa and adipose stromal cells are associated with comparable changes in cytochrome P-450(AROM) gene expression and synthesis, whereas the reductase component is only modestly affected. Studies are in progress to define the molecular mechanisms involved in the regulation of cytochrome P-450(AROM) gene expression in estrogen-produced cells.

AB - Aromatase, an enzyme complex localized in the endoplasmic reticulum of estrogen-producing cells, is composed of NADPH-cytochrome P-450 reductase, and aromatase cytochrome P-450 (cytochrome P-450(AROM)). To define the molecular mechanisms involved in the multifactorial regulation of cytochrome P-450(AROM) in estrogen-producing cells, we have isolated a cDNA specific for human cytochrome P-450(AROM) and have used this cDNA to isolate the human cytochrome P-450(AROM) gene. The cDNA sequence encodes a polypeptide of 503 amino acids and contains - near the carboxy-terminus, a region of high homology with the putative heme-binding regions of other P-450 cytochromes. COS1 cells transfected with an expression plasmid containing the cytochrome P-450(AROM) cDNA had the capacity to aromatize testosterone, androstenedione and 16α-hydroxyandrostenedione, suggesting that a single polypeptide catalyzes all steps of the aromatization reaction using either of the three major C19-substrates. The human cytochrome P-450(AROM) gene is > 52 kb in size and consists of 10 exons and 9 introns. Hormonally induced changes in aromatase activity of human ovarian granulosa and adipose stromal cells are associated with comparable changes in cytochrome P-450(AROM) gene expression and synthesis, whereas the reductase component is only modestly affected. Studies are in progress to define the molecular mechanisms involved in the regulation of cytochrome P-450(AROM) gene expression in estrogen-produced cells.

UR - http://www.scopus.com/inward/record.url?scp=0025219853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025219853&partnerID=8YFLogxK

M3 - Article

C2 - 1690030

AN - SCOPUS:0025219853

VL - 42

SP - 1

EP - 10

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 1

ER -