Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway

Chao Tong, Jin Jiang

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

The Hedgehog (Hh)-signaling pathway has been intensively studied in the past decade. Increasing evidence suggests that dynamic formation of protein complexes plays a critical role in the organization and regulation of Hh signaling. Immunoprecipitation (IP) is a powerful tool to study protein-protein interactions and has provided important insights into the regulation of Hh signal transduction. Here, we show how to use IP to study protein-protein interactions in the Drosophila Hh-signaling pathway.

Original languageEnglish (US)
Title of host publicationHedgehog Sigaling Protocols
Pages215-229
Number of pages15
DOIs
StatePublished - Sep 13 2007

Publication series

NameMethods in Molecular Biology
Volume397
ISSN (Print)1064-3745

Keywords

  • Epitope tag
  • Hedgehog-signaling pathway
  • Immunoprecipitation
  • Protein A
  • Protein G

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway'. Together they form a unique fingerprint.

  • Cite this

    Tong, C., & Jiang, J. (2007). Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway. In Hedgehog Sigaling Protocols (pp. 215-229). (Methods in Molecular Biology; Vol. 397). https://doi.org/10.1385/1-59745-516-4:215