Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway

Chao Tong, Jin Jiang

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

The Hedgehog (Hh)-signaling pathway has been intensively studied in the past decade. Increasing evidence suggests that dynamic formation of protein complexes plays a critical role in the organization and regulation of Hh signaling. Immunoprecipitation (IP) is a powerful tool to study protein-protein interactions and has provided important insights into the regulation of Hh signal transduction. Here, we show how to use IP to study protein-protein interactions in the Drosophila Hh-signaling pathway.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages215-229
Number of pages15
Volume397
DOIs
StatePublished - Sep 13 2007

Publication series

NameMethods in Molecular Biology
Volume397
ISSN (Print)10643745

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Immunoprecipitation
Proteins
Signal Transduction

Keywords

  • Epitope tag
  • Hedgehog-signaling pathway
  • Immunoprecipitation
  • Protein A
  • Protein G

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Tong, C., & Jiang, J. (2007). Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway. In Methods in Molecular Biology (Vol. 397, pp. 215-229). (Methods in Molecular Biology; Vol. 397). https://doi.org/10.1385/1-59745-516-4:215

Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway. / Tong, Chao; Jiang, Jin.

Methods in Molecular Biology. Vol. 397 2007. p. 215-229 (Methods in Molecular Biology; Vol. 397).

Research output: Chapter in Book/Report/Conference proceedingChapter

Tong, C & Jiang, J 2007, Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway. in Methods in Molecular Biology. vol. 397, Methods in Molecular Biology, vol. 397, pp. 215-229. https://doi.org/10.1385/1-59745-516-4:215
Tong C, Jiang J. Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway. In Methods in Molecular Biology. Vol. 397. 2007. p. 215-229. (Methods in Molecular Biology). https://doi.org/10.1385/1-59745-516-4:215
Tong, Chao ; Jiang, Jin. / Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway. Methods in Molecular Biology. Vol. 397 2007. pp. 215-229 (Methods in Molecular Biology).
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