Abstract
The interaction of macromolecules with themselves and with other macromolecules is fundamental to the functioning of living systems. Recent advances in the analysis of sedimentation velocity (SV) data obtained by analytical ultracentrifugation allow the experimenter to determine important features of such interactions, including the equilibrium association constant and information about the kinetic off-rate of the interaction. The determination of these parameters is made possible by the ability of modern software to fit numerical solutions of the Lamm Equation with kinetic considerations directly to SV data. Herein, the SV analytical advances implemented in the software package SEDPHAT are summarized. Detailed analyses of SV data using these strategies are presented. Finally, a few highlights of recent literature reports that feature this type of SV data analysis are surveyed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4-15 |
| Number of pages | 12 |
| Journal | Methods |
| Volume | 54 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 2011 |
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Keywords
- Analytical ultracentrifugation
- Lamm-Equation modeling
- Sedimentation velocity
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Using Lamm-Equation modeling of sedimentation velocity data to determine the kinetic and thermodynamic properties of macromolecular interactions. / Brautigam, Chad A.
In: Methods, Vol. 54, No. 1, 05.2011, p. 4-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Using Lamm-Equation modeling of sedimentation velocity data to determine the kinetic and thermodynamic properties of macromolecular interactions
AU - Brautigam, Chad A
PY - 2011/5
Y1 - 2011/5
N2 - The interaction of macromolecules with themselves and with other macromolecules is fundamental to the functioning of living systems. Recent advances in the analysis of sedimentation velocity (SV) data obtained by analytical ultracentrifugation allow the experimenter to determine important features of such interactions, including the equilibrium association constant and information about the kinetic off-rate of the interaction. The determination of these parameters is made possible by the ability of modern software to fit numerical solutions of the Lamm Equation with kinetic considerations directly to SV data. Herein, the SV analytical advances implemented in the software package SEDPHAT are summarized. Detailed analyses of SV data using these strategies are presented. Finally, a few highlights of recent literature reports that feature this type of SV data analysis are surveyed.
AB - The interaction of macromolecules with themselves and with other macromolecules is fundamental to the functioning of living systems. Recent advances in the analysis of sedimentation velocity (SV) data obtained by analytical ultracentrifugation allow the experimenter to determine important features of such interactions, including the equilibrium association constant and information about the kinetic off-rate of the interaction. The determination of these parameters is made possible by the ability of modern software to fit numerical solutions of the Lamm Equation with kinetic considerations directly to SV data. Herein, the SV analytical advances implemented in the software package SEDPHAT are summarized. Detailed analyses of SV data using these strategies are presented. Finally, a few highlights of recent literature reports that feature this type of SV data analysis are surveyed.
KW - Analytical ultracentrifugation
KW - Lamm-Equation modeling
KW - Sedimentation velocity
UR - http://www.scopus.com/inward/record.url?scp=79955609636&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955609636&partnerID=8YFLogxK
U2 - 10.1016/j.ymeth.2010.12.029
DO - 10.1016/j.ymeth.2010.12.029
M3 - Article
C2 - 21187153
AN - SCOPUS:79955609636
VL - 54
SP - 4
EP - 15
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - 1
ER -