Using surface-bound rubidium ions for protein phasing

S. Korolev, I. Dementieva, R. Sanishvili, W. Minor, Z. Otwinowski, A. Joachimiak

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Rubidium is a monovalent metal that can be used as a counterion in protein solutions. X-ray anomalous scattering from rubidium ions bound to the protein surface was used for phasing of the crystal structure of the hsp60 apical domain from Thermus thermophilus. Multiple-wavelength anomalous dispersion (MAD) data were collected from a crystal obtained from a solution containing 0.2 M rubidium salt. One molecule of protein (147 amino acids) binds one well ordered and one poorly ordered Rb atom. Phases calculated with the program SHARP were sufficient for automatic tracing and side-chain assignment using the program ARP/wARP. The data show that bound rubidium ions can be used to determine protein structures and to study the interaction of monovalent metal ions with proteins and other macromolecules.

Original languageEnglish (US)
Pages (from-to)1008-1012
Number of pages5
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number7
DOIs
StatePublished - Jul 19 2001

ASJC Scopus subject areas

  • Structural Biology

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