Utilization of transferrin-bound iron by Haemophilus influenzae requires an intact tonB gene

G. P. Jarosik, I. Maciver, E. J. Hansen

Research output: Contribution to journalComment/debate

27 Scopus citations

Abstract

Haemophilus influenzae can utilize iron-loaded human transferrin as an iron source for growth in vitro. H. influenzae tonB mutants, containing a chloramphenicol acetyltransferase gene within their tonB genes, could bind iron-charged human transferrin to their cell surfaces, but they were unable to utilize this serum glycoprotein as the sole source of iron for growth in vitro. In contrast, these tonB mutants were able to utilize an iron chelate (ferric ammonium citrate) for growth. Transformation of a tonB mutant with a plasmid encoding a wild-type H. influenzae tonB gene restored the ability of a tonB mutant to utilize iron-charged human transferrin. These results indicate that the uptake of iron from human transferrin by H. influenzae is a TonB-dependent process.

Original languageEnglish (US)
Pages (from-to)710-713
Number of pages4
JournalInfection and immunity
Volume63
Issue number2
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Utilization of transferrin-bound iron by Haemophilus influenzae requires an intact tonB gene'. Together they form a unique fingerprint.

  • Cite this