Uv-RNA immunoprecipitation (Uv-rip) protocol in neurons

Katie Schaukowitch, Jae Yeol Joo, Tae Kyung Kim

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

With the many advances in genome-wide sequencing, it has been discovered that much more of the genome is transcribed into RNA than previously appreciated. These nonprotein-coding RNAs (ncRNAs) come in many different forms, and they have been shown to have a variety of functions within the cell, infl uencing processes such as gene expression, mRNA splicing, and transport, just as a few examples. As we delve deeper into studying their mechanisms of action, it becomes important to understand how they play these roles, in particular by understanding what proteins these ncRNAs interact with. This protocol describes one technique that can be used to study this, ultra-violet light cross-linking RNA immunoprecipitation (UV-RIP), which uses an antibody to pull down a specifi c protein of interest and then detects RNA that is bound to it. This technique utilizes UV light to cross-link the cells, which takes advantage of the fact that UV light will only cross-link proteins and nucleic acids that are directly interacting. This approach can provide key mechanistic insight into the function of these newly identifi ed ncRNAs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages33-38
Number of pages6
Volume1468
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1468
ISSN (Print)10643745

Keywords

  • LncRNA
  • RNA immunoprecipitation
  • RNA-binding proteins
  • UV cross-linking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Uv-RNA immunoprecipitation (Uv-rip) protocol in neurons'. Together they form a unique fingerprint.

Cite this