UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism

Georg Schuhmachers, Kiyoshi Ariizumi, Toshiyuki Kitajima, Dale Edelbaum, Shan Xu, Richard K. Shadduck, Gary L. Gilmore, R. Stan Taylor, Paul R. Bergstresser, Akira Takashima

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9 Scopus citations

Abstract

We have established long-term dendritic cell lines from the epidermis of newborn mice. These cell lines (XS series) proliferate maximally in response to granulocyte/macrophage-colony stimulating factor, as well as to CSF-1, which is produced by skin-derived NS fibroblast lines and by keratinocytes (albeit in smaller amounts). The purpose of this study was to examine the impact of UVB radiation on CSF-1-mediated interaction of dendritic cells with fibroblasts and keratinocytes. Exposure of NS cells to UVB radiation (unfiltered FS20 sunlamp) decreased CSF-1 production at mRNA and protein levels. Both changes occurred in a dose-dependent fashion, with 50 J/m2 causing a significant reduction. UVB radiation also downregulated CSF-1 mRNA expression by Pam 212 keratinocytes. UVB exposure of XS cells diminished the surface expression of CSF-1 receptors, with 50 J/m2 causing a significant reduction. Thus, UVB radiation interrupts CSF-1-mediated cell-cell interaction by a dual mechanism: downregulating CSF-1 production and abrogating CSF-1 receptor expression. Importantly, granulocyte/macrophage-colony stimulating factor receptor expression by XS cells was also inhibited by UVB radiation, once again, with 50 J/m2 producing significant inhibition. We propose that the resulting CSF-1 deficiency in epidermal microenvironment and unresponsiveness by dendritic cells to relevant growth factors may contribute to UVB-mediated loss of resident epidermal dendritic cells (i.e., Langerhans cells) in skin.

Original languageEnglish (US)
Pages (from-to)1023-1029
Number of pages7
JournalJournal of Investigative Dermatology
Volume106
Issue number5
DOIs
StatePublished - Jan 1 1996

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Schuhmachers, G., Ariizumi, K., Kitajima, T., Edelbaum, D., Xu, S., Shadduck, R. K., Gilmore, G. L., Taylor, R. S., Bergstresser, P. R., & Takashima, A. (1996). UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism. Journal of Investigative Dermatology, 106(5), 1023-1029. https://doi.org/10.1111/1523-1747.ep12338592