UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism

Georg Schuhmachers, Kiyoshi Ariizumi, Toshiyuki Kitajima, Dale Edelbaum, Shan Xu, Richard K. Shadduck, Gary L. Gilmore, R. Stan Taylor, Paul R. Bergstresser, Akira Takashima

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Abstract

We have established long-term dendritic cell lines from the epidermis of newborn mice. These cell lines (XS series) proliferate maximally in response to granulocyte/macrophage-colony stimulating factor, as well as to CSF-1, which is produced by skin-derived NS fibroblast lines and by keratinocytes (albeit in smaller amounts). The purpose of this study was to examine the impact of UVB radiation on CSF-1-mediated interaction of dendritic cells with fibroblasts and keratinocytes. Exposure of NS cells to UVB radiation (unfiltered FS20 sunlamp) decreased CSF-1 production at mRNA and protein levels. Both changes occurred in a dose-dependent fashion, with 50 J/m2 causing a significant reduction. UVB radiation also downregulated CSF-1 mRNA expression by Pam 212 keratinocytes. UVB exposure of XS cells diminished the surface expression of CSF-1 receptors, with 50 J/m2 causing a significant reduction. Thus, UVB radiation interrupts CSF-1-mediated cell-cell interaction by a dual mechanism: downregulating CSF-1 production and abrogating CSF-1 receptor expression. Importantly, granulocyte/macrophage-colony stimulating factor receptor expression by XS cells was also inhibited by UVB radiation, once again, with 50 J/m2 producing significant inhibition. We propose that the resulting CSF-1 deficiency in epidermal microenvironment and unresponsiveness by dendritic cells to relevant growth factors may contribute to UVB-mediated loss of resident epidermal dendritic cells (i.e., Langerhans cells) in skin.

Original languageEnglish (US)
Pages (from-to)1023-1029
Number of pages7
JournalJournal of Investigative Dermatology
Volume106
Issue number5
StatePublished - 1996

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Macrophage Colony-Stimulating Factor
Langerhans Cells
Radiation
Cytokines
Cell Line
Macrophage Colony-Stimulating Factor Receptors
Keratinocytes
Dendritic Cells
Fibroblasts
Skin
Down-Regulation
Granulocyte-Macrophage Colony-Stimulating Factor Receptors
Messenger RNA
Granulocyte-Macrophage Colony-Stimulating Factor
Epidermis
Cell Communication
Intercellular Signaling Peptides and Proteins
Cells

ASJC Scopus subject areas

  • Dermatology

Cite this

Schuhmachers, G., Ariizumi, K., Kitajima, T., Edelbaum, D., Xu, S., Shadduck, R. K., ... Takashima, A. (1996). UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism. Journal of Investigative Dermatology, 106(5), 1023-1029.

UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism. / Schuhmachers, Georg; Ariizumi, Kiyoshi; Kitajima, Toshiyuki; Edelbaum, Dale; Xu, Shan; Shadduck, Richard K.; Gilmore, Gary L.; Taylor, R. Stan; Bergstresser, Paul R.; Takashima, Akira.

In: Journal of Investigative Dermatology, Vol. 106, No. 5, 1996, p. 1023-1029.

Research output: Contribution to journalArticle

Schuhmachers, G, Ariizumi, K, Kitajima, T, Edelbaum, D, Xu, S, Shadduck, RK, Gilmore, GL, Taylor, RS, Bergstresser, PR & Takashima, A 1996, 'UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism', Journal of Investigative Dermatology, vol. 106, no. 5, pp. 1023-1029.
Schuhmachers, Georg ; Ariizumi, Kiyoshi ; Kitajima, Toshiyuki ; Edelbaum, Dale ; Xu, Shan ; Shadduck, Richard K. ; Gilmore, Gary L. ; Taylor, R. Stan ; Bergstresser, Paul R. ; Takashima, Akira. / UVB radiation interrupts cytokine-mediated support of an epidermal-derived dendritic cell line (XS52) by a dual mechanism. In: Journal of Investigative Dermatology. 1996 ; Vol. 106, No. 5. pp. 1023-1029.
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abstract = "We have established long-term dendritic cell lines from the epidermis of newborn mice. These cell lines (XS series) proliferate maximally in response to granulocyte/macrophage-colony stimulating factor, as well as to CSF-1, which is produced by skin-derived NS fibroblast lines and by keratinocytes (albeit in smaller amounts). The purpose of this study was to examine the impact of UVB radiation on CSF-1-mediated interaction of dendritic cells with fibroblasts and keratinocytes. Exposure of NS cells to UVB radiation (unfiltered FS20 sunlamp) decreased CSF-1 production at mRNA and protein levels. Both changes occurred in a dose-dependent fashion, with 50 J/m2 causing a significant reduction. UVB radiation also downregulated CSF-1 mRNA expression by Pam 212 keratinocytes. UVB exposure of XS cells diminished the surface expression of CSF-1 receptors, with 50 J/m2 causing a significant reduction. Thus, UVB radiation interrupts CSF-1-mediated cell-cell interaction by a dual mechanism: downregulating CSF-1 production and abrogating CSF-1 receptor expression. Importantly, granulocyte/macrophage-colony stimulating factor receptor expression by XS cells was also inhibited by UVB radiation, once again, with 50 J/m2 producing significant inhibition. We propose that the resulting CSF-1 deficiency in epidermal microenvironment and unresponsiveness by dendritic cells to relevant growth factors may contribute to UVB-mediated loss of resident epidermal dendritic cells (i.e., Langerhans cells) in skin.",
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AU - Edelbaum, Dale

AU - Xu, Shan

AU - Shadduck, Richard K.

AU - Gilmore, Gary L.

AU - Taylor, R. Stan

AU - Bergstresser, Paul R.

AU - Takashima, Akira

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AB - We have established long-term dendritic cell lines from the epidermis of newborn mice. These cell lines (XS series) proliferate maximally in response to granulocyte/macrophage-colony stimulating factor, as well as to CSF-1, which is produced by skin-derived NS fibroblast lines and by keratinocytes (albeit in smaller amounts). The purpose of this study was to examine the impact of UVB radiation on CSF-1-mediated interaction of dendritic cells with fibroblasts and keratinocytes. Exposure of NS cells to UVB radiation (unfiltered FS20 sunlamp) decreased CSF-1 production at mRNA and protein levels. Both changes occurred in a dose-dependent fashion, with 50 J/m2 causing a significant reduction. UVB radiation also downregulated CSF-1 mRNA expression by Pam 212 keratinocytes. UVB exposure of XS cells diminished the surface expression of CSF-1 receptors, with 50 J/m2 causing a significant reduction. Thus, UVB radiation interrupts CSF-1-mediated cell-cell interaction by a dual mechanism: downregulating CSF-1 production and abrogating CSF-1 receptor expression. Importantly, granulocyte/macrophage-colony stimulating factor receptor expression by XS cells was also inhibited by UVB radiation, once again, with 50 J/m2 producing significant inhibition. We propose that the resulting CSF-1 deficiency in epidermal microenvironment and unresponsiveness by dendritic cells to relevant growth factors may contribute to UVB-mediated loss of resident epidermal dendritic cells (i.e., Langerhans cells) in skin.

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