Vasopressin regulation of the proestrous luteinizing hormone surge in wild-type and Clock mutant mice

Brooke H. Miller, Susan Losee Olson, Jon E. Levine, Fred W. Turek, Teresa H. Horton, Joseph S. Takahashi

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

In the female mouse, ovulation and estrous cyclicity are under both hormonal and circadian control. We have shown that mice with a mutation in the core circadian gene Clock have abnormal estrous cycles and do not have a luteinizing hormone (LH) surge on the afternoon of proestrus due to a defect at the hypothalamic level. In the present study, we tested the hypotheses that vasopressin (AVP) can act as a circadian signal to regulate the proestrous release of LH, and that this signal is deficient in the Clock mutant. We found that Avp expression in the suprachiasmatic nucleus (SCN) and AVP 1a receptor (Avpr1a) expression in the hypothalamus is reduced in Clock mutant mice compared to wild-type mice. Intracerebroventricular (i.c.v.) injection of AVP on the afternoon of proestrus is sufficient to induce LH secretion, which reaches surge levels in 50% of Clock mutant mice. The effect of AVP on the Clock mutant LH surge is mediated by AVPR1A, as co-infusion of AVP and an AVPR1A-specific antagonist prevents AVP induction of LH release, although infusion of an AVPR1A antagonist into wild-type mice failed to prevent a proestrous LH surge. These results suggest that reduced hypothalamic AVP signaling plays a role in the absence of the proestrous LH surge in Clock mutant mice. The results also support the hypothesis that AVP produced by the SCN may be a circadian signal that regulates LH release.

Original languageEnglish (US)
Pages (from-to)778-784
Number of pages7
JournalBiology of Reproduction
Volume75
Issue number5
DOIs
StatePublished - Nov 2006

Fingerprint

Luteinizing Hormone
Vasopressins
Proestrus
Suprachiasmatic Nucleus
Circadian Clocks
Estrous Cycle
Periodicity
Ovulation
Hypothalamus
Mutation
Injections
Genes

Keywords

  • Circadian rhythm
  • Gonadotropin-releasing hormone
  • Luteinizing hormone
  • Ovulatory cycle
  • Vasopressin

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Vasopressin regulation of the proestrous luteinizing hormone surge in wild-type and Clock mutant mice. / Miller, Brooke H.; Olson, Susan Losee; Levine, Jon E.; Turek, Fred W.; Horton, Teresa H.; Takahashi, Joseph S.

In: Biology of Reproduction, Vol. 75, No. 5, 11.2006, p. 778-784.

Research output: Contribution to journalArticle

Miller, Brooke H. ; Olson, Susan Losee ; Levine, Jon E. ; Turek, Fred W. ; Horton, Teresa H. ; Takahashi, Joseph S. / Vasopressin regulation of the proestrous luteinizing hormone surge in wild-type and Clock mutant mice. In: Biology of Reproduction. 2006 ; Vol. 75, No. 5. pp. 778-784.
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