Vertebrate kidney tubules elongate using a planar cell polarity-dependent, rosette-based mechanism of convergent extension

Soeren S. Lienkamp, Kun Liu, Courtney M. Karner, Thomas J. Carroll, Olaf Ronneberger, John B. Wallingford, Gerd Walz

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Cystic kidney diseases are a global public health burden, affecting over 12 million people1. Although much is known about the genetics of kidney development and disease, the cellular mechanisms driving normal kidney tubule elongation remain unclear2,3. Here, we used in vivo imaging to show for the first time that mediolaterally oriented cell intercalation is fundamental to vertebrate kidney morphogenesis. Unexpectedly, we found that kidney tubule elongation is driven in large part by a myosin-dependent, multicellular rosette-based mechanism, previously only described in Drosophila melanogaster. In contrast to findings in Drosophila, however, non-canonical Wnt and planar cell polarity (PCP) signaling is required to control rosette topology and orientation during vertebrate kidney tubule elongation. These data resolve long-standing questions concerning the role of PCP signaling in the developing kidney and, moreover, establish rosette-based intercalation as a deeply conserved cellular engine for epithelial morphogenesis.

Original languageEnglish (US)
Pages (from-to)1382-1387
Number of pages6
JournalNature Genetics
Volume44
Issue number12
DOIs
StatePublished - Dec 2012

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Kidney Tubules
Cell Polarity
Vertebrates
Morphogenesis
Cystic Kidney Diseases
Kidney
Kidney Diseases
Myosins
Drosophila melanogaster
Drosophila
Public Health

ASJC Scopus subject areas

  • Genetics

Cite this

Vertebrate kidney tubules elongate using a planar cell polarity-dependent, rosette-based mechanism of convergent extension. / Lienkamp, Soeren S.; Liu, Kun; Karner, Courtney M.; Carroll, Thomas J.; Ronneberger, Olaf; Wallingford, John B.; Walz, Gerd.

In: Nature Genetics, Vol. 44, No. 12, 12.2012, p. 1382-1387.

Research output: Contribution to journalArticle

Lienkamp, Soeren S. ; Liu, Kun ; Karner, Courtney M. ; Carroll, Thomas J. ; Ronneberger, Olaf ; Wallingford, John B. ; Walz, Gerd. / Vertebrate kidney tubules elongate using a planar cell polarity-dependent, rosette-based mechanism of convergent extension. In: Nature Genetics. 2012 ; Vol. 44, No. 12. pp. 1382-1387.
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