Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods

M. B. Carpenter, L. Chang, A. B. Pereira, L. B. Hersh, G. Bruce, J. Y. Wu

Research output: Contribution to journalArticle

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Abstract

Attempts were made to identify vestibular (VEN) and cochlear (CEN) efferent neurons in the squirrel monkey using retrograde transport of horseradish peroxidase (HRP) and immunocytochemical methods. HRP implants in the ampulla of the lateral semicircular duct retrogradely labeled cells of VEN bilaterally and some cells of CEN. VEN located lateral to the rostral part of the abducens nucleus formed a compact collection of cells, all of which were immunoreactive only to antisera for choline acetyltransferase (ChAT). CEN, identified by immunoreactivity to ChAT were located at the hilus of the lateral superior olive (LSO), along the lateral border of the LSO and sparsely near lateral parts of the ventral trapezoid nucleus (VTN). A small number of cells and fibers near the border of the VTN and lateral to the LSO were immunoreactive for leucine enkephalin (L-ENK). Fibers immunoreactive for L-ENK also were identified in the hilus of the LSO. No cells of the superior olivary complex were immunoreactive for antisera to ChAT, L-ENK, substance P, γ-aminobutyric acid or glutamic acid decarboxylase. Cells of VEN and CEN can be identified by their immunoreactivity to ChAT, and some cells and fibers of CEN also contain L-ENK.

Original languageEnglish (US)
Pages (from-to)275-280
Number of pages6
JournalBrain Research
Volume408
Issue number1-2
DOIs
StatePublished - Apr 7 1987

Fingerprint

Efferent Neurons
Cochlea
Haplorhini
Leucine Enkephalin
Choline O-Acetyltransferase
Horseradish Peroxidase
Immune Sera
Semicircular Ducts
Ventral Thalamic Nuclei
Aminobutyrates
Saimiri
Glutamate Decarboxylase
Substance P
Cell Count
Superior Olivary Complex

Keywords

  • Abducens nucleus
  • Choline acetyltransferase
  • Leucine enkephalin
  • Olivocochlear bundle
  • Periolivary nucleus
  • Superior olivary complex
  • Vestibular efferent neuron

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods. / Carpenter, M. B.; Chang, L.; Pereira, A. B.; Hersh, L. B.; Bruce, G.; Wu, J. Y.

In: Brain Research, Vol. 408, No. 1-2, 07.04.1987, p. 275-280.

Research output: Contribution to journalArticle

Carpenter, M. B. ; Chang, L. ; Pereira, A. B. ; Hersh, L. B. ; Bruce, G. ; Wu, J. Y. / Vestibular and cochlear efferent neurons in the monkey identified by immunocytochemical methods. In: Brain Research. 1987 ; Vol. 408, No. 1-2. pp. 275-280.
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abstract = "Attempts were made to identify vestibular (VEN) and cochlear (CEN) efferent neurons in the squirrel monkey using retrograde transport of horseradish peroxidase (HRP) and immunocytochemical methods. HRP implants in the ampulla of the lateral semicircular duct retrogradely labeled cells of VEN bilaterally and some cells of CEN. VEN located lateral to the rostral part of the abducens nucleus formed a compact collection of cells, all of which were immunoreactive only to antisera for choline acetyltransferase (ChAT). CEN, identified by immunoreactivity to ChAT were located at the hilus of the lateral superior olive (LSO), along the lateral border of the LSO and sparsely near lateral parts of the ventral trapezoid nucleus (VTN). A small number of cells and fibers near the border of the VTN and lateral to the LSO were immunoreactive for leucine enkephalin (L-ENK). Fibers immunoreactive for L-ENK also were identified in the hilus of the LSO. No cells of the superior olivary complex were immunoreactive for antisera to ChAT, L-ENK, substance P, γ-aminobutyric acid or glutamic acid decarboxylase. Cells of VEN and CEN can be identified by their immunoreactivity to ChAT, and some cells and fibers of CEN also contain L-ENK.",
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